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Xiangdong Zhou



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    P2.03 - Biology (Not CME Accredited Session) (ID 952)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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      P2.03-24 - CD90 Enhances Metastasis by Epithelial-Mesenchymal Transdifferentiation in Lung Adenocacinoma (ID 13271)

      16:45 - 18:00  |  Presenting Author(s): Xiangdong Zhou

      • Abstract

      Background

      The specific mechanism of lung cancer remains unclear. The existence of cancer stem cells（CSCs） reflects the cellular heterogeneity within tumors,and we presume that CD90pos lung adenocarcinoma(LA) cells play the role of CSCs.

      The epithelial-to-mesenchymal transition (EMT) is a de-differentiation process that has been implicated in metastasis and generation.In this study, CD90pos CSC were isolated and characterized from LA cell line A549, and we discovered that the specific subtype displayed CSCs and epithelial-mesenchymal transition phenotypes.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Section not applicable

      4c3880bb027f159e801041b1021e88e8 Result

      1. CD90pos CSC was isolated and characterized from LA cell line A549.

      1)FACS assay was utilized to isolated CD90pos cells from LA cell line A549 ,we found that adherent A549 cells possess much lower proportion of CD90 than A549 sphere cells, indicating that CD90 maybe the potential CSCs marker.

      2) CD90pos cells expressed higher stemness genes Oct4 and Nanog via Western blot analysis. Also the clonal formation and sphere formation assay by CD90pos and CD90neg cells was performed, and the former highly expressed proliferation capability.

      3) The tumorigenicity assay in nude mice showed CD90pos cells highly expressed tumorigenicity in vivo, which possessed shorter latency stage and formed larger xenograft nodes.

      2. CD90pos cells possessed highly metastasis and invasiveness associated with epithelial mesenchymal transition.

      1) Migrative and invasive ability of CD90pos cells was measured by migration and Transwell invasion assay, and we found that CD90pos cells with higher migrative and invasive ability in vitro compared with CD90neg cells.

      2) To further confirm upper observation, an experimental tail vein metastasis model was performed in vivo,and CD90pos cells displayed a superior ability to metastasize to the lung.

      3) RT-PCR measurement of gene expression related with EMT showed same tendency compared with Western blot analysis and immunofluorescence assay.MMP2 was higher expression in CD90pos cells, also transcription factors Twist increased significantly, in which mould be involved in the EMT phenotype maintaining.

      3. CD90 expression has important clinical significance for patients with LA.

      8eea62084ca7e541d918e823422bd82e Conclusion

      CD90 is a effective marker for isolation and enrichment of LCSCs. CD90pos cells highly expressed stem cell related genes, self-renewal capability in vitro, showed stronger tumorigenicity in vivo. Furthermore, CD90pos cells possesse higher metastasis and invasiveness, which is associated with EMT.CD90pos cells are endowed with EMT phenotype and show an decreased expression of E-cadherin, and increased expression of N-cadherin, vimentin, MMP2, which would be associated with Twist. Expression of CD90 is negatively correlated with survival time in the metastatic sites instead of primary sites.

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    P3.01 - Advanced NSCLC (Not CME Accredited Session) (ID 967)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall
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      P3.01-115 - Liposomal Paclitaxel Versus Gemcitabine: Which is Better? (ID 13299)

      12:00 - 13:30  |  Presenting Author(s): Xiangdong Zhou

      • Abstract

      Background

      Lung cancer tends to metastasize to lymph nodes in the early stage.The liposomal Paclitaxel(LP) injection has less side effects and higher uptake.It is also characterized as being able to target to organs with rich reticuloendothelial system and keeping a high concentration for a long time. We assume that LP injection has a better curative effect against NSCLC with regional lymph node metastasis.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Patients divide to 58 for LP and 56 for Gemcitabine plus cisplatin (GP).Those patients have good comparability in gender,age,ECOG points,pathological pattern,TNM staging and chemotherapy cycles.21 days as a single cycle, each patient finished at least 2 cycles. Estimation was carried on within one week when they have finished the chemotherapy. Chemotherapy Continues if the result turns out CR+PR+SD. surgical treatment would be suggested if the stage of cancer decreases and the lesions of NSCLC turn technically removable. Then continue chemotherapy after surgery, 4 to 6 cycles in total.

      4c3880bb027f159e801041b1021e88e8 Result

      The alleviation rate of lymph nodes metastasis in LP group is significantly higher.The disease control rate of lymph nodes metastasis is 96.551% vs. 92.857%, p=0.38.The alleviation rate of squamous cell carcinoma and the disease control rate is not statistically significant. The alleviation and disease control rate of primary tumor in LP and GP group does not have statistical significance.And among LA patients,it the same trend.
      The LP group has a longer period without disease progression and a higher survival rate in 1 year than the GPgroup.The medium PFS of squamous cell carcinoma in 5 LP group is 8 months which is higher than that in GP group (5.5 months) but does not have statistical significance. The survival rate in 1 year of squamous cell is 50% (16/32).

      The PFS of IIIB stage in LP group is 10 months, significantly higher than GP group(4 months),survival rate in 1 year in LP group is also higher but is not statistically significant.

      As to side effect, the LP group is significantly lower in the incidence of thrombocytopenia, granulocytopenia, anemia and white blood cell reduction.However, the LP group is higher incidence of alopecia.

      8eea62084ca7e541d918e823422bd82e Conclusion

      Chemotherapy with LP has higher alleviation rate than chemotherapy with GP in treating advanced NSCLC with regional lymph node metastasis, especially for squamous cell carcinoma. The survival rate and PFS in stage IIIB/IV of LP is also higher than that of GP. Meanwhile, LP is able to reduce the incidence rate of side effects such as hrombocytopenia, granulocytopenia,anemia, white blood cell reduction, nausea and vomiting.

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