Author of

• 20156

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  P1.07 - Immunology and Immunotherapy (ID 693)

  • Event: WCLC 2017
  • Type: Poster Session with Presenters Present
  • Track: Immunology and Immunotherapy
  • Presentations: 1
  • Moderators:
  • Coordinates: 10/16/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)

  • 22689

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    P1.07-021 - Multiplex Immune Profiling Identifies Prognostic Importance of the Spatial Co-Localization of Immune Cells in NSCLC (ID 9419)

    09:30 - 16:00  |  Presenting Author(s): Artur Mezheyeuski
    • Abstract
    • Slides

    Background:
    There is an increasing impact of the immunotherapy in the treatment of NSCLC. The accurate characterization of immune cell infiltrates in the in situ environment of cancer is regarded to be of major importance to predict prognosis and to guide therapy. The aim of this study was to perform a multi-marker characterization of immune cells and to evaluate their spatial distribution patterns with regard to patient survival.
    
    Method:
    A tissue microarray including 55 cases of non-small cell lung cancer (NSCLC) was used to perform multiplex immuno-fluorescent staining with antibodies against CD8, CD20, CD4, FoxP3, CD45RO and pan-cytokeratin (Opal, Perkin Elmer). The staining was digitalized by a multispectral imaging system (Vectra 3, PerkinElmer). Single cell marker expression profiles and their tissue coordinates were used to evaluate cell quantity and spatial distribution patterns. The data were validated with conventional immunohistochemical staining on consecutive sections.
    
    Result:
    Based on the co-expression of single immune markers we determined eight different classes of immune cells. While CD8+ single positive cells are evenly distributed in the stroma and tumor cell compartment, CD20+ and CD4+ cells were predominantly located in the stroma. Based on the immune cell subtypes we could define patients with a predominant B-cell response and patient with dominating T-cell infiltrates. No statistically significant impact on survival was found with regard to the abundance of immune cell subpopulations. When the spatial relation of stromal CD8+ regulatory T cells (CD8+FoxP3+) was considered, the shorter distance (< 20 μm) between CD8+FoxP3+ cells and tumor cells was associated with shorter survival (median 34 vs. 76 month, HR=2.6, 95%CI 1.3-6.8, log-rank p<0.01).
    
    Conclusion:
    The fluorescence multiplexed IHC technique provides a multi-marker characterization and spatial information for single cell-level analysis of immune infiltration patterns. Spatial localization of CD8+ regulatory T lymphocytes in relation to cancer cells is associated with overall survival in NSCLC.
    
    

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