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Yiying Guo
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P47 - Small Cell Lung Cancer/NET - Biology / Translational (ID 234)
- Event: WCLC 2020
- Type: Posters
- Track: Small Cell Lung Cancer/NET
- Presentations: 1
- Moderators:
- Coordinates: 1/28/2021, 00:00 - 00:00, ePoster Hall
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P47.09 - Quantification and Classification of Tumor Infiltrating Leukocytes (TILs) and its Impact on Prognosis in Small Cell Lung Cancer (SCLC) (ID 3804)
00:00 - 00:00 | Presenting Author(s): Yiying Guo
- Abstract
Introduction
Small cell lung cancer (SCLC) is a refractory malignancy with little improvement in treatment over 30 years. Recently, immunotherapy has improved survival only in a small subset of SCLC patients. Lacking of insight for tumor microenvironment in SCLC has made a hurdle for expanding the beneficiaries.
Methods
Forty-eight SCLC patients with detailed clinical data and complete follow-up information were included. The proportion of TILs was estimated on hematoxylin and eosin (H&E)-stained tumor sections. Transcriptome profile was detected based on Nanostring nCounter platform. By applying CIBERSORT, we assessed the relative proportions of 22 types of immune cells in tumor tissues. The prognostic effect of specific TILs and differentially expressed immune-related genes were evaluated respectively grouped by TILs semi-quantification.
Results
Patients were divided into two groups by the estimated abundance of TILs at the optimal cut-off of 30% with 12 TIL-high and 36 TIL-low patients. TIL-high group suffered a worse disease-free survival (DFS) and overall survival (OS) (5-year DFS%, 16.7% vs. 54.2%, P=0.015; hazard ratio, [HR] 2.55 P=0.019, 5-year OS%, 33.3% vs. 68.1%, P=0.001; HR 3.93 P=0.002). Both groups had hyporesponsive immune cells in the tumor microenvironment (TME). Among the detected immune-related mRNAs, IL-6 was up-regulated in the TME of patients with high immune infiltrates (log fold change=0.837, P=0.116). High level of IL6 and high abundance of the Treg cells were independent unfavorable factors for OS (IL6, 5-year OS%, 41.3% vs. 81.0%, HR=10.76, P<0.001; Treg cells, 5-year OS%, 0% vs. 19.4%, HR=6.86, P=0.002).
Conclusion
This study showed that high abundance of TILs was not always associated better survival and the impact of TILs’ abundance on prognosis varied depending on the cytological type and functional status of the immune cell subsets (ICSs) infiltrated in TME. Besides, targeting the Treg cells and IL-6 might be effective to overcome the immunosuppressive TME in SCLC for patients with high enrichment level of these factors.
