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Mi Hyoung Sophia Moon



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    P62 - Tumor Biology and Systems Biology - Basic and Translational Science - Metabolomics (ID 200)

    • Event: WCLC 2020
    • Type: Posters
    • Track: Tumor Biology and Systems Biology - Basic and Translational Science
    • Presentations: 1
    • Moderators:
    • Coordinates: 1/28/2021, 00:00 - 00:00, ePoster Hall
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      P62.01 - 3D Cell Model Development for Optimizing Intrapleural Hyperthermic Chemotherapy in Advanced Lung Cancer (ID 2174)

      00:00 - 00:00  |  Presenting Author(s): Mi Hyoung Sophia Moon

      • Abstract
      • Presentation

      Introduction

      Malignant pleural effusion as a result of pleural seeding of primary lung cancer or primary malignant pleural mesothelioma (MPM) has shown poor prognosis with median survival 6 to 18 months. There is no standard treatment yet, but intrapleural hyperthermic chemotherapy (IPHC) in combination with surgery or systemic chemotherapy has been tried for palliative local control. However, the optimal treatment condition (temperature, duration, type and dose of chemotherapeutic agent) has not known yet. We developed 3D lung cancer and mesothelioma model to reflect the intrapleural cavity, and tested different combinations of temperature, exposure duration, and concentration of the cisplatin to assess best combination of the intrapleural hyperthermic chemotherapy.

      Methods

      Aiming to reflect intrapleural environment, we developed 3D cell strain model by using 3D cell culture system derived of human blood (ProtinetTM, DaNAgreen©, Rep. of Korea). Commercially available cell lines (A549, NCI-H2170, NCI-H28) were obatined from Korea Cell Line Bank. Cells were cultured in Dulbecco's Modified Eagle's Medium at 37℃ in a humidified atmosphere containing 5% CO2.

      For exploring optimal IPHC condition, we tested different temperatures (37℃ (control), 43℃, 45℃), duration of exposure (5min, 15 min, 30 min), and concentration of cisplatin (0 ~ 100 uM). Different combinations are treated, and the cell models were incubated in the same condition for 48 hours and then LDH assay was done.

      Results

      For temperature, 45'C was the most effective compared to other conditions (Figure 1).

      figure1.png

      For concentration and expsoure time, LDH cytotoxicity assay revealed 50uM of cisplatin and 15 min of exposure time under 45'C was most effective (Figure 2).

      figure 2.png

      Conclusion

      The new 3D cell culture system made from human serum (Protinet) was effective and durable for repeated experiment. With the 3D tumor model, 45'C, 15 min exposure and 50 uM of cisplatin was most effective. Validation with patient-derived 3D model is ongoing currently.

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