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Diana Monserrat Aguilar-Beltrán



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    P46 - Screening and Early Detection - Gene-Based Risk Stratification (ID 183)

    • Event: WCLC 2020
    • Type: Posters
    • Track: Screening and Early Detection
    • Presentations: 1
    • Moderators:
    • Coordinates: 1/28/2021, 00:00 - 00:00, ePoster Hall
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      P46.03 - DNA Methylation Analysis in Smokers and Non-Smokers (Passive and ex-Smokers) (ID 1435)

      00:00 - 00:00  |  Presenting Author(s): Diana Monserrat Aguilar-Beltrán

      • Abstract
      • Slides

      Introduction

      Each year, tobacco's consumption increases worldwide in young people and in Mexico 30% of consumers begin their addiction around age 12. Smoking in Querétaro has increased more than the national average and this confers a risk because the epigenetic changes that can appear and be inherited; like DNA methylation in nucleotides found in sites called CpGs islands located in the promoters of the genes, which, when modified, directly affect their transcriptional processes. When this mechanism is aberrant as in hypomethylation, genes such as oncogenes can be activated, but hypermethylation can cause the silencing of genes, such as tumor suppressors or those responsible for DNA repair. Therefore, the purpose of the project was to establish the percentage of global DNA methylation in peripheral blood caused by tobacco consumption, to find their association as an early marker of lung cancer.

      Methods

      Peripheral blood samples were obtained with EDTA of students between 18 and 24 years, after signing an informed consent, were classified as non-smokers, ex-smokers, smokers and passive depending on cigarettes or hours of exposure. While patients with lung cancer, had an age range of 56-84 years. The Methylated DNA 5-mC Methyl Flash kit was used to quantify the global DNA methylation through an indirect ELISA.

      Results

      Averages in the percentage of global DNA methylation were 0.32% in non-smokers, 0.36% in passive smokers, 0.66% in smokers, 0.97% in ex-smokers and 1.58% in lung cancer patients. All the exposed groups showed elevated levels of global DNA methylation, even the ex-smokers. After performing one-way ANOVA analysis, it was observed a significant statistical difference between the groups (p<0.05).

      Conclusion

      This finding suggests, based on data on age, number of cigarettes, exposure period, etc; that the percentages or major changes in DNA methylation are due to the dose and / or prolonged exposure to cigarette smoke. However, the ex-smokers group keep showing high levels of methylation, this could provide help in starting to classify the early risk in the percentage of methylated DNA in peripheral blood.

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