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Lixin Zhou



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    FP07 - Pathology (ID 109)

    • Event: WCLC 2020
    • Type: Posters (Featured)
    • Track: Pathology, Molecular Pathology and Diagnostic Biomarkers
    • Presentations: 1
    • Moderators:
    • Coordinates: 1/28/2021, 00:00 - 00:00, ePoster Hall
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      FP07.03 - Landscape Heterogeneity of PD-L1 Expression and Immune Cells Predicts Prognosis of Metastatic Non-Small Cell Lung Cancer (ID 878)

      00:00 - 00:00  |  Author(s): Lixin Zhou

      • Abstract
      • Presentation
      • Slides

      Introduction

      Immune microenvironment evolution may occur during tumor progression. The purpose of this study is to quantitatively access the heterogeneity and its significance of PD-L1 expression and immune cells (ICs) during the metastatic progression of non-small cell lung cancer (NSCLC).

      Methods

      Matched 64 resected specimens of paired primary tumors (PTs) and metastatic tumors (MTs) were obtained from 28 NSCLC patients. PD-L1 co-expression on tumor cells (TCs) and ICs (CD8+ cytotoxic T cells and CD68+ macrophages) as well as the density and spatial distance of ICs were analyzed by DNA-tagged multiplex staining on whole sections using HALO@ platform. The prognostic significance of the heterogeneity between MTs and corresponding PTs were concerned.

      Results

      Heterogeneity in the tumor proportion score (TPS) of PD-L1 expression on TCs, density of ICs, and spatial distance between ICs and TCs was observed between MTs and PTs. Importantly, subgroup analysis showed that significant discrepancies of TPS and ICs between MTs and PTs were associated with treatment (Figure 1). It was also novelty found that the alterations in PD-L1 expression on ICs during metastasis were closely associated with overall survival (OS) in NSCLC. Compared to corresponding PTs, an increase in TPS on MTs was slightly related to poor prognosis, while an increase in the density of PD-L1+ ICs and decrease in spatial distance between PD-L1+/CD8+ T cells and TCs was significantly correlated with prolonged OS as a positive immune response (Figure 2).

      Figure 1. Represented multiplex figures and quantitative analysis of the heterogeneity between MTs and PTs, Wilcoxon-signed-rank-test.

      figure 1.png

      Figure 2. The change status of PD-L1 expression and ICs on MTs compared to corresponding PTs was closely related with overall survival (OS).

      figure 2.png

      Conclusion

      Landscape of PD-L1 expression and ICs in MT and corresponding PT to highlight the change status of immune microenvironment predicts prognosis of metastatic NSCLC.

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