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Yuko Iida
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P38 - Pathology - Pathology/Staging (ID 108)
- Event: WCLC 2020
- Type: Posters
- Track: Pathology, Molecular Pathology and Diagnostic Biomarkers
- Presentations: 1
- Moderators:
- Coordinates: 1/28/2021, 00:00 - 00:00, ePoster Hall
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P38.09 - Clinicopathological Characteristics of Combined Small-Cell Lung Cancer (ID 3643)
00:00 - 00:00 | Presenting Author(s): Yuko Iida
- Abstract
Introduction
Small-cell lung cancer (SCLC) includes pure SCLC, which involves a single type of tissue, and combined small-cell lung cancer (cSCLC), which also includes non-small-cell lung cancer (NSCLC). The molecular biological characteristics of cSCLC have not been sufficiently elucidated thus far. In this study, we have examined the clinicopathological and molecular biological characteristics of cSCLC cases.
Methods
We focused on patients with limited-stage cSCLC who underwent surgery at our hospital from 2010 to 2019. Using their medical records, we performed a retrospective clinical study. From the formalin-fixed paraffin-embedded (FFPE) tissues, DNA and RNA of the cSCLC cases were extracted for each tissue type. By performing immunostaining for neuroendocrine markers for all the cases and next-generation sequencing using the DNA of each tissue type for the cSCLC cases, we examined the somatic gene mutations related to lung cancer. Further, we quantified the mRNA expression levels of ASCL1, INSM1, BRN2, and NF1B by RT-PCR.
Results
Four cases of cSCLC was confirmed. The median age of the cSCLC cases was 76 (range: 60–81) years, the gender ratio was three males to one female, and the mixed histological types were as follows: two cases of adenocarcinoma and two cases of squamous-cell carcinoma. In the cSCLC cases, common gene mutations, such as TP53 and RB1 gene mutations, were found in both the SCLC and NSCLC components. Furthermore, the expression of ASCL1 was significantly higher in the SCLC component than in the NSCLC component.
Conclusion
Since the common gene mutations were found in the SCLC and NSCLC components of the cSCLC cases, SCLC component and NSCLC component are believed to share a common origin. Moreover, it is considered that the strength of the expression of ASCL1 might be involved in the differences between each tissue type.
