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Zheng Ma



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    P34 - Pathology - Liquid Biopsy (ID 104)

    • Event: WCLC 2020
    • Type: Posters
    • Track: Pathology, Molecular Pathology and Diagnostic Biomarkers
    • Presentations: 1
    • Moderators:
    • Coordinates: 1/28/2021, 00:00 - 00:00, ePoster Hall
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      P34.09 - The Dynamic Changes of ctDNA and cfDNA in the Perioperative Period of Early NSCLC have the Potential to Predict the Risk of Recurrence (ID 1937)

      00:00 - 00:00  |  Presenting Author(s): Zheng Ma

      • Abstract
      • Slides

      Introduction

      Circulation tumor DNA (ctDNA) is the part of cell free DNA (cfDNA) that comes from the tumor, which can be used for precise treatment and prediction of postoperative recurrence risk of NSCLC. However, the amount of ctDNA in patients with early NSCLC, especially postoperative, is too small to allow accurate measurement, which greatly reduces the sensitivity of detection and increases false negative. On the other hand, cfDNA is mainly derived from cell damage, and the large amount of cell damage caused during the surgery leads to a significant increase in cfDNA. Since the half-life of cfDNA is very short, whether the amount of cfDNA can be restored to the preoperative level in a short time after surgery may indicate the degree of the body's response to the traumatic stress, and thus has the potential to be a predictor of recurrence risk. In this study, dynamic changes of ctDNA and cfDNA in the perioperative period of NSCLC were used to explore the possibility of ctDNA and cfDNA as biomarkers indicating the risk of recurrence.

      Methods

      NSCLC patients who planned to undergo radical resection were screened. 10ml of peripheral blood was collected before, during and 7 days after surgery, and intraoperative tissues were also taken as controls. Real-time fluorescent quantitative PCR was used to measure the content of cfDNA, and a 23-gene NGS panel was performed to detect tumor tissue and monitor the abundance of ctDNA.

      Results

      A total of 7 patients were sampled from March to August 2019. The sensitivity and specificity of preoperative blood and tissue by NGS detection were 43.75% and 100%, respectively. Only 1 patient tested positive for ctDNA in postoperative blood. The amount of cfDNA before surgery was 36.6±14.7ng, and increased to 127.2±52.2ng during surgery. However, cfDNA was polarized 7 days after surgery: 3 patients showed a significant decline, and basically recovered to the preoperative level of 45.23±9.41ng; the other 4 cases continued to rise to 173.7±80.80ng. So far, one patient with negative postoperative ctDNA and continuously elevated cfDNA has a recurrence.

      Conclusion

      Early NSCLC has very low ctDNA level, which may be false-negative for postoperative recurrence risk assessment. The cfDNA levels are raised by the operation, but will be polarized after 7 days. Patients with perioperative elevated cfDNA may have a higher risk of recurrence.

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