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EP1.03 - Biology (ID 193)
- Event: WCLC 2019
- Type: E-Poster Viewing in the Exhibit Hall
- Track: Biology
- Presentations: 1
- Now Available
- Coordinates: 9/08/2019, 08:00 - 18:00, Exhibit Hall
EP1.03-29 - The Evaluation of Non-Canonical WNT/β-Catenin Ligands Expression Status in Non-Small Cell Lung Cancer (Now Available) (ID 2528)
08:00 - 18:00 | Author(s): Radosław Charkiewicz
The family of secreted glycoproteins forming the WNT/β-catenin signaling pathway is shown to play a significant role in many types of carcinomas including NSCLC. Therefore, the aim of the study was to evaluate the WNT pathway status in NSCLC.Method
The study was performed on 80 pairs of cancerous and non-cancerous tissue samples and 5 NSCLC cell lines (A549, H1299, H1395, Calu-3, H520 and non-cancerous control line HBEpC). Selected WNT genes expression analysis was assessed with qPCR with TaqMan Gene Expression Assays. The relative expression of chosen genes was estimated with ΔΔCt method. Statistical analyses were performed with the use of: Shapiro-Wilk test, T-test Wilcoxon matched pairs singed-rank test and Mann-Whitney U test.Result
In the cell lines deregulation of most selected genes was detected. Fold-change analysis of obtained results has shown a down-regulation of WNT4, -5A, -7A and -11 expression in comparison to control. However, in H520 and A549 cell lines up-regulation of WNT-5A and WNT -11 was found.
Analysis of patient’s tissue shows statistically significant (p < 0.05) inhibition of non-canonical WNT pathway ligands WNT-4, WNT-5A, WNT-7A and WNT-11 in cancerous tissue in comparison to non-cancerous tissue. In adenocarcinoma statistically significant dysregulation of WNT5A (p = 0.0094), -7A (p < 0.0001), and -11 (p < 0.0001) was identify. In squamous cell carcinoma statistically significant dysregulation of WNT4 (p , 0.0001), -7A (p < 0.0001) and -11 (p < 0.0001) was found. Moreover, in large cell carcinoma statistically significant dysregulation of WNT-4 (p = 0.0068), -5A (p = 0.0210), -7A (p = 0.0156) and -11 (p = 0.0117) was discovered. Furthermore, a statistically significant difference of WNT-7A expression between stage IA and IIA tumors (p = 0.0203) has been found.Conclusion
Dysregulation of non-canonical WNT/β-catenin signaling pathway ligands expression profile in NSCLC tissue samples suggests that it play an important role in NSCLC. Further studies, are necessary to proof the mechanisms of WNT/β-catenin pathway in NSCLC.