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EP1.03 - Biology (ID 193)
- Event: WCLC 2019
- Type: E-Poster Viewing in the Exhibit Hall
- Track: Biology
- Presentations: 1
- Now Available
- Coordinates: 9/08/2019, 08:00 - 18:00, Exhibit Hall
EP1.03-26 - Analysis of Gene Expression Profiling of Lung Adenocarcinoma Brain Metastasis (Now Available) (ID 775)
08:00 - 18:00 | Author(s): Sun-Yong Jun
The molecular biology of lung adenocarcinoma (ADC) brain metastasis is poorly understood. We aimed to explore genes that are implicated in the process of brain metastasis of primary lung adenocarcinoma.Method
Twelve primary lung ADC with brain metastasis (ADCBM), six non-metastatic lung ADC (NMADC) and six lung ADC with metastasis except in the brain (ADCEB) were selected. Samples from all primary lung ADC were obtained after surgical resections performed at Incheon St. Mary’s hospital. All tumors were analyzed with the NanoString nCounter system for gene expression using the nCounter PanCancer Progression panel composed of 740 genes. Expression data were analyzed using the NanoString nSolver software ver 4.0 and nCouter Advanced Analysis ver 2.0.115.Result
Eleven out of the 740 genes were differentially expressed between the ADCBM and ADC without brain metastasis including NMADC and ADCEB. The genes that were downregulated in the ADCBM included RGCC, ACHE, TPSB2, and FHL1. Expression levels of EPHB3, AGRN, ISLR, TNFRSF12A, TPM2, PTRF and BGN were upregulated in the ADCBM compared to ADC without brain metastasis including NMADC and ADCEB. The ADCBM was compared with NMADC and ADCEB, respectively. Four genes were differentially expressed between the ADCBM and NMADC. 23 genes were differentially expressed between the ADCBM and ADCEB. The differentially expressed genes (DEGs) in NMADC vs. ADCEB were not identified the same DEGs in ADCBM vs. NMADC and ADCEB. As a result, the EPHB3, AGRN and TNFRSF12A that were upregulated and the RGCC that was downregulated in ADCBM was common identified.Conclusion
Conclusion: Although our findings are not conclusive, we have identified differentially expressed genes that might mediate the brain metastasis process. A prospective validation is needed to confirm candidate genes associated with ADCBM.
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