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  EP1.03 - Biology (ID 193)

  • Event: WCLC 2019
  • Type: E-Poster Viewing in the Exhibit Hall
  • Track: Biology
  • Presentations: 1
  • Now Available
  • Moderators:
  • Coordinates: 9/08/2019, 08:00 - 18:00, Exhibit Hall

  • 31914

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    EP1.03-20 - Diagnostic and Prognostic Utility of Differentially Expressed Circulating MicroRNAs in Indian NSCLC Patients (Now Available) (ID 875)

    08:00 - 18:00  |  Author(s): Ashraf Ali
    • Abstract
    • Slides

    Background
    

    The presence of circulating tumour DNA, mRNA and non-coding RNAs, such as microRNA (miRNA), in the serum and plasma of cancer patients has sparked great interest because conventional diagnostic tests tend to be imperfect and more invasive, posing logistic difficulties for serial tumour sampling. Hence, identification of differentially expressed circulating miRNAs in the serum of non-small cell lung cancer (NSCLC) patients may have potential as cancer biomarkers for diagnosis, prognosis and predicting therapeutic response.

    Method
    

    For the identification of differentially expressed miRNAs in the serum of NSCLC patients, we performed small RNA sequencing with illumina HiSeq 2000 platform (n=10; 4 NSCLC patients and 6 controls). The expression profile of miRNAs in each subject was analyzed using miRNAkey software and fold change was performed to identify differentially expressed miRNAs in NSCLC as compared to controls. We validated the expression of few differentially expressed miRNAs in the serum of 75 NSCLC patients and 40 controls using miScript qRT-PCR assay. The expression of miRNAs was correlated with overall survival (OS), progression-free survival (PFS), response to therapy and various clinico-pathological parameters.

    Result
    

    The mean age of NSCLC patients and controls was 56.2 years and 55.3 years, respectively (p = 0.3242). Majority of the NSCLC patient and controls were male. 67% of NSCLC patients and 53% of controls were smokers (p = 0.099). Global miRNA profiling revealed 16 differentially expressed miRNAs (cut-off: fold change > 2.0, or p < 0.05, or both) in the serum of NSCLC patients as compared to controls. Our qRT-PCR data revealed significant down-regulation of miR-15a-5p, miR-320a, miR-25-3p, miR-192-5p, let-7d-5p, let-7e-5p, miR-148a-3p and miR-92a-3p in the serum of NSCLC patients as compared to controls. None of the miRNAs were correlated with survival outcome and therapeutic response. The expression of miR-320a, miR-25-3p and miR-148a-3p significantly correlated with stage, while miR-375 expression significantly correlated with lymph node involvement and pleural effusion.

    Conclusion
    

    The expression of majority of miRNAs was down-regulated in the serum of NSCLC patients as compared to controls. Some of the miRNAs, such as miR-375 and miR-320a, are less studied for their involvement in the pathogenesis of NSCLC. Hence, future mechanistic studies are warranted to elucidate their role in disease biology and as candidate biomarkers for diagnosis and prognosis of NSCLC.

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