Author of

• 31891

  +

  EP1.03 - Biology (ID 193)

  • Event: WCLC 2019
  • Type: E-Poster Viewing in the Exhibit Hall
  • Track: Biology
  • Presentations: 1
  • Now Available
  • Moderators:
  • Coordinates: 9/08/2019, 08:00 - 18:00, Exhibit Hall

  • 31908

    +

    EP1.03-15 - Comparison of Plasma Expression of Drosha and Dicer mRNAs in Early and Advanced Stages of NSCLC Patients (Now Available) (ID 628)

    08:00 - 18:00  |  Author(s): Grażyna Balicka
    • Abstract
    • Slides

    Background
    

    Drosha and Dicer are the enzymes necessary during the miRNA biogenesis. They have the same effect on all microRNAs. Many studies have focused on profiling the expression of selected miRNAs or whole miRNom’s analysis in serum or plasma, as potential tool for early detection of diseases. We would like to check, whether the expression of Drosha and Dicer mRNA is detectable in plasma of NSCLC patients, and whether it can differentiate early and advanced stages of this disease.

    Method
    

    We enrolled 59 (43.1%) NSCLC patients in early (I-IIIA) stages and 78 (56.9%) in locally advanced or advanced (IIIB-IV) stages. We isolated total mRNA and reverse transcription PCR (RT-PCR) was performed. RT-PCR was made using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Real-time PCR (qPCR) was performed for assessment of Drosha and Dicer mRNA expression on Eco Illumina Real-Time PCR system device (Illumina Inc.). We used TaqMan probe Hs00203008_m1 (Applied Biosystems) for Drosha and Hs00229023_ m1 (Applied Biosystems) for Dicer mRNA expression measurement. GAPDH was used a housekeeping gene. Statistical analysis were performed with use of Statistica 13.1 software (Tibco).

    Result
    

    Drosha mRNA expression was detectable in plasma of 57 (41.6%) patients. 17 (28.8%) patients with Drosha mRNA expression were in early stages and 40 (51.3%) patients were in stages IIIB or IV (χ²=6.98, p=0.008).

    Expression of Dicer mRNA was detectable in plasma of 71 (57.8%) patients. 15 (25.4%) of patients from this group were in early stages and 56 (71.8%) – in IIIB or IV stages (χ²=28.93, p<0.0001).

    Significant higher expression of Drosha mRNA was observed in group of patients with lymph node involvement compared with group of patients without lymph node metastases (p=0.001).

    Moreover, significantly higher expression of Dicer mRNA was observed in group of patients with distant metastases compared with group without metastases (p=0.0002).

    Furthermore, we found statistically nonsignificant (p=0.07) lower expression of Drosha mRNA in stages IIIB-IV compared with early stages.

    We did not find any differences between Drosha or Dicer mRNAs expression in patients stratified by age, tumor size or histopathological diagnosis (p<0.1).

    Conclusion
    

    Plasma expression of Drosha and Dicer mRNAs is detected more often in advanced stages of NSCLC. Probably, different mRNAs from more damaged tumor cells in more advanced disease stages are present in higher expression in blood stream. However, this proves that free mRNAs of Drosha and Dicer are mainly produced by cancer cells in NSCLC patients. indirectly, it can be concluded that cancer cells have disturbed production of microRNAs. There are necessity to use more sensitive tools (i.e. Next Generation Sequencing method) to asses expression of Dicer and Drosha mRNAs in early stages of NSCLC.

    Only Active Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login or select "Add to Cart" and proceed to checkout.