Virtual Library

Start Your Search

Sho Horiuchi



Author of

  • +

    P1.04 - Immuno-oncology (ID 164)

    • Event: WCLC 2019
    • Type: Poster Viewing in the Exhibit Hall
    • Track: Immuno-oncology
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/08/2019, 09:45 - 18:00, Exhibit Hall
    • +

      P1.04-54 - Inter-Tumor Heterogeneity of PD-L1 Expressions in Non-Small Cell Lung Cancer (ID 48)

      09:45 - 18:00  |  Author(s): Sho Horiuchi

      • Abstract
      • Slides

      Background

      Following to approval of Pembrolizumab for patients with advanced NSCLC, PD-L1 IHC 22C3 pharmDx (Dako) was adopted as a companion diagnostic test. While PD-L1 IHC 28-8 pharmDx (Dako) was established as a complementary diagnostic for Nivolumab. Recently many groups demonstrated the intra-tumor heterogeneity of these PD-L1 expressions, but there have been a few reports about the intra-patient or inter-tumor heterogeneity. We aimed to investigate the inter-tumor heterogeneity of PD-L1 IHC 22C3 and 28-8 pharmDx (Dako).

      Method

      Between December 1, 2014 and May 7, 2018, total 517 patients with NSCLC underwent surgical resection at our hospital. We excluded all patients with no informed consent, with no lymph node metastasis, with chemotherapy/radiotherapy before surgery and with never enough volume of material for genetic testing. Finally 35 formalin-fixed paraffin-embedded primary tumors with paired metastatic lymph nodes were available in this study. After staining by PD-L1 IHC 22C3 and 28-8 pharmDx (Dako) respectively, we counted tumor cells exhibiting membrane staining and calculated Tumor Proportion Score (TPS). Afterward, all cases were classified into three subgroups as follows; No Expression (TPS: <1%), Low Expression (TPS: 1-49%) and High Expression (TPS: ≥50%).

      Result

      Average age of 35 patients was 66.8 years old and there were 10 females (28.6%), 10 never smokers (28.6%), 27 adenocarcinomas (77.1%) and 11 tumors with EGFR mutation. The number of cases in No Expression, Low Expression and High Expression in 22C3 were 7 (20.0%), 22 (62.8%) and 6 (17.1%) in primary tumor, meanwhile 18 (51.4%), 13 (37.1%) and 4 (11.4%) in metastatic lymph node, respectively. The concordant rate was 28.6% between TPS subgroups in primary tumor and that in metastatic lymph node. About 28-8 antibody, No Expression, Low Expression and High Expression were 8 (22.9%), 21 (60.0%) and 6 (17.1%) in primary tumor, meanwhile 18 (51.4%), 11 (31.4%) and 6 (17.1%) in metastatic lymph node, respectively. The concordant rate was 31.4% between TPS subgroups in primary tumor and that in metastatic lymph node.

      Conclusion

      Our result demonstrated apparent discrepancy of TPS between primary tumor and metastatic lymph node in both PD-L1 IHC 22C3 and 28-8.

      Only Active Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login or select "Add to Cart" and proceed to checkout.

  • +

    P2.11 - Screening and Early Detection (ID 178)

    • Event: WCLC 2019
    • Type: Poster Viewing in the Exhibit Hall
    • Track: Screening and Early Detection
    • Presentations: 1
    • Now Available
    • Moderators:
    • Coordinates: 9/09/2019, 10:15 - 18:15, Exhibit Hall
    • +

      P2.11-17 - Analysis of Lung Adenocarcinoma EGFR Mutation by LAMP Method: Comparison with PCR Method and Identification of a Novel Exon19 Deletion Mutation (Now Available) (ID 69)

      10:15 - 18:15  |  Presenting Author(s): Sho Horiuchi

      • Abstract
      • Slides

      Background

      Detection of EGFR mutation has been widely used for the lung cancer treatment. The accuracy of detecting EGFR mutations is, therefore, very important. We here adopted a method of Loop-mediated isothermal amplification (LAMP) used for the detection of bacteria, which amplifies DNA with high specificity and efficiency under isothermal conditions. So that we evaluated the usefulness of LAMP for detecting various EGFR mutations using cancer tissues in comparison to conventional PCR technique.

      Method

      We enrolled 59 surgically resected lung adenocarcinoma patients. We used Therascreen EGFR PCR Kit as a conventional PCR. Then, we tried to detect EGFR mutations for those specimens using LAMP method, and compared the result of LAMP to that of Therascreen.

      Result

      26 cases had no mutation in the analysis using Therascreen and LAMP method. In 32 cases which showed positive mutations according to Therascreen, and LAMP method, however, we found a single case showing no mutations by LAMP method, and exon 19 deletion by Threascreen. The direct sequence analysis revealed no mutations in the case as shown with LAMP method. Here, we repeated several experiments using LAMP method for the purpose of comfirmation of EGFR status of the case. These additional tests revealed Exon 19 deletion using the LAMP method, moreover, another direct sequencing discovered a novel EGFR mutation. Sensitivity for LAMP method was calculated as 97.0%, specificity as 100%, positive predictive value (PPV) was 100%, negative predictive value (NPV) was 96.3 % and accuracy was 98.3%.

      Conclusion

      The accuracy of detecting EGFR mutation in LAMP method was nealy equivalent to that in Therascreen. Moreover, we detected a novel EGFR exon 19 deletion mutation with the direct sequence.

      Only Active Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login or select "Add to Cart" and proceed to checkout.