Author of

• 30292

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  EP1.01 - Advanced NSCLC (ID 150)

  • Event: WCLC 2019
  • Type: E-Poster Viewing in the Exhibit Hall
  • Track: Advanced NSCLC
  • Presentations: 1
  • Now Available
  • Moderators:
  • Coordinates: 9/08/2019, 08:00 - 18:00, Exhibit Hall

  • 30351

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    EP1.01-53 - Update of Mutation Status in Lung Cancer. A Multicenter Local Study (Now Available) (ID 2466)

    08:00 - 18:00  |  Author(s): Maximiliano Franco Canigiani
    • Abstract
    • Slides

    Background
    

    Advances have been made in the understanding of the biology of NSCLC in relation to the characterization of molecular features such as activations of oncogenes by mutations, translocations and amplifications, which are being used as a targets and predictive biomarkers. Molecular analysis of NSCLC, adenocarcinoma (AC) is now the standard of care for therapy selection.

    Method
    

    We determined the molecular alterations in EGFR , and gene fusion ALK in our Caucasian and Hispanic populations

    169 small samples and resection specimens of patients with NSCLC in different institutions of Cordoba were studied during a period (2014 - 2019).

    In addition to Histopathology Type, we analyzed immunohistochemistry (IHC) characteristics, molecular profiles , and several clinical variables were studied.

    Different tests were used to detect alterations of EGFR and fusion gene EML4-ALK expression, with the aim to identify our own profile. EGFR mutation was studied by therascreen kit, PCR, in order to detect genetic alterations in exons 18, 19, 20 and 21. ALK translocations were analyzed by FISH (Vysis- Break Apart, Abbott) and IHC (clon D5F3, ventana, Roche). We correlated the molecular profile with different clinical variables (age, gender, and tobacco habits). The statistical method used was the multiple regression logistic model.

    Result
    

    169 samples were tested for EGFR expression and alk alterations 64% of subjects were men and 89% were smokers. Smoking habit was associated with sex (33% and 67% of smokers were women and men respectively, p=0.009). Smokers were older than non-smokers (p=0.011); to 64.4 (0.98) and 57.5 (2.35) years old for smokers and non-smokers.

    EGFR alterations were associated with sex (p=0.002, Fig 1). Women had more chance of having positive alterations of the gene (OR 3.57, 95CI:2.04-7.55). Age and smoking habit of patients did not show significant effects (p=0.44, Fig.2 , and p=0.09, respectively).

    Only 1.7% of subjects reported ALK alterations, and were not related to sex (p=0.42), age (p=0.965) and smoking habit (p=0.281).

    Conclusion
    

    Our results showed a comparable frequency in EGFR mutations and gene fusion ALK in relation to the data published in western population. These results allowed a proper diagnosis to provide the most adequate therapy.

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