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Junji Ichinose



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    MA18 - Advances in Diagnosis of Common Types of NSCLC (ID 145)

    • Event: WCLC 2019
    • Type: Mini Oral Session
    • Track: Pathology
    • Presentations: 1
    • Now Available
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      MA18.03 - Distinction Between Primary Lung Cancer and Pulmonary Metastasis of Esophageal Cancer Using the Nanostring nCounter System (Now Available) (ID 1228)

      11:30 - 13:00  |  Presenting Author(s): Junji Ichinose

      • Abstract
      • Presentation
      • Slides

      Background

      It is very difficult to distinguish between primary lung squamous cell carcinoma (LSCC) and pulmonary metastasis of esophageal squamous cell carcinoma (ESCC) in patients with past history of ESCC, even by histological examination of the resected specimen. This study aimed to distinguish between LSCC and metastasis of ESCC by multiplex gene expression analysis using the Nanostring nCounter analysis system.

      Method

      RNA was extracted from the FFPE samples of eight LSCCs, thirteen ESCCs, and nine indeterminate SCCs in the lung of patients with history of ESCC. We selected ten genes which were differently expressed markedly between LSCC and ESCC using the nCounter PanCancer Pathways Panel (XT-CSO-PATH1-12). We performed linear discriminant analysis between the two groups. The derived discriminant function was applied to the nine indeterminate SCCs. The nCounter diagnosis was compared to the preoperative features, pathological findings and postoperative prognosis.

      Result

      Four of nine pulmonary tumors were diagnosed as LSCC and five were diagnosed as metastasis of ESCC. None of four patients with LSCC died and one developed recurrence, while all of five patients with metastasis of ESCC died and all developed recurrence. The prognosis of the latter was significantly poorer than the former (logrank test, p = 0.02). The preoperative features which indicate metastasis of ESCC (multiple lesions, short disease-free interval and presence of local recurrence) were found only in the metastasis group.

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      Conclusion

      Multiplex gene expression analysis using the nCounter was useful for discrimination between LSCC and pulmonary metastasis of ESCC.

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