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George G Chen



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    Lunch & Poster Display session (ID 58)

    • Event: ELCC 2019
    • Type: Poster Display session
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 4/11/2019, 12:30 - 13:00, Hall 1
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      19P - FOXP3Δ3, the major isoform of FOXP3, promotes proliferation, migration, and invasion in non-small cell lung cancer (ID 515)

      12:30 - 13:00  |  Author(s): George G Chen

      • Abstract

      Background

      Increasing evidence has shown that FOXP3 is not only expressed in immune cells but also in tumor cells. However, the results of tumor FOXP3 is inconsistent and even the opposite. Unlike mice, humans express multiple isoforms of FOXP3. However, the expression and role of FOXP3 isoforms in cancers are still largely unknown. The role of FOXP3 and FOXP3Δ3 in NSCLC remains unclear.

      a9ded1e5ce5d75814730bb4caaf49419 Methods

      The expression of FOXP3 and four FOXP3 isoforms was analyzed using the RSEM method for 32 cancer types from TCGA databases. The correlation between the expression of FOXP3 and prognosis of NSCLC patients was analyzed (http://kmplot.com/). Western blots assay, colony formation assay, MTT assay, wound-healing assay, and cell invasion assay were performed to determine the cell functions and to measure the expression of related molecules after the ectopic expression of FOXP3 and FOXP3Δ2 in NSCLC cells.

      20c51b5f4e9aeb5334c90ff072e6f928 Results

      The expression of FOXP3 was upregulated in NSCLC tissues compared to adjacent non-tumor lung tissues. The FOXP3Δ3 was the most frequent FOXP3 isoform detected in NSCLC as well as in almost all types of cancers. The high expression of FOXP3 was correlated with poor prognosis in NSCLC. Tumor cells with FOXP3Δ3 were much more oncogenic than those without, and the ectopic expression of either FOXP3FL or FOXP3Δ3 could promote the proliferation, migration, and invasion of A549 and H23 cells. The ectopic expression of FOXP3 and FOXP3Δ3 downregulated E-cadherin and upregulated N-cadherin, snail, slug, MMP2, and MMP7.

      fd69c5cf902969e6fb71d043085ddee6 Conclusions

      FOXP3Δ3 is the major isoform of FOXP3 in NSCLC and it may be a predictive factor for NSCLC patients.

      b651e8a99c4375feb982b7c2cad376e9 Legal entity responsible for the study

      The Chinese University of Hong Kong.

      213f68309caaa4ccc14d5f99789640ad Funding

      Research Grants Council of the Hong Kong SAR (No: CUHK462613) and the National Natural Science Foundation of China (No: 81472742).

      682889d0a1d3b50267a69346a750433d Disclosure

      All authors have declared no conflicts of interest.

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