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X. Hong

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    Poster Session (ID 8)

    • Event: ACLC 2018
    • Type: Poster Session
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 11/07/2018, 00:00 - 00:00, Poster Hall
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      P052 - A Biomarker Study of Advanced Non-Small Cell Lung Cancer Using Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (ID 177)

      00:00 - 00:00  |  Author(s): X. Hong

      • Abstract

      This study is to explore changes of serum metabolites in patients with advanced non-small cell lung cancer (NSCLC) and healthy controls, so as to provide new diagnostic strategies for NSCLC.

      A total of 151 samples were obtained, including 86 NSCLC patients and 65 healthy controls. After serum was extracted, metabolic assays based on a wide range of targeted metabolome technologies and the Ultra-High-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS /MS) detection platform were applied to detect metabolites in it. A self-built database and the multivariate statistical analysis were utilized to explore the difference in metabolome between the two arms.

      A total of 296 metabolites were detected in all samples. Eighty-three genes were found differentially expressed, of which 20 were down-regulated and 63 were up-regulated in NSCLC patients. There were no significant differences in the serum metabolite between adenocarcinoma and squamous cell carcinoma. KEGG functional annotation analysis was made on the 81 metabolites to elucidate their biological functions. Signaling pathways which might cause changes in metabolites were summarized.

      Based on our novel UPLC-MS / MS platform, more metabolites can be detected, which might provide new potential tumor biomarkers. Our study can accelerate development of new diagnostic strategies in NSCLC.