Author of

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  P2.03 - Biology (Not CME Accredited Session) (ID 952)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall

  • 27896

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    P2.03-05 - Biologic Profiling of Brain Metastasis from Non-Small Cell Lung Cancer (ID 13080)

    16:45 - 18:00  |  Author(s): Masaki Kaibori
    • Abstract
    • Slides

    Background
    

    Bain metastasis develops in approximately 50% of patients with non-small cell lung cancer (NSCLC), resulting in poor prognosis and low health-related quality of life. Immunological microenvironment and functions of ion channels have been indicated to play pertinent roles in epithelial‐to‐mesenchymal transition (EMT) and its reverse mesenchymal‐to‐epithelial transition (MET), crucial steps in distant metastasis, but their actual roles in brain metastasis of NSCLC remains unclear. The aim of this study is to investigate biologic profiles of brain metastasis of NSCLC.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    Formalin-fixed paraffin-embedded (FFPE) samples of brain metastasis and paired primary sites were collected from 3 patients with NSCLC undergoing surgical resection between Jan. 2012 and Dec. 2017. Total RNA was extracted from the archived FFPE, of which integrity was briefly checked by Nanodrop. Gene expression was detected by nCounter (NanoString Technologies, WA, USA) with PanCancer Immune Profilling Panel and custom-made KMU IonChannel Panel that covered selected 100 genes. Detected data was analyzed by nCounter Advanced Analysis (version 2.0.115). Factors with unadjusted P-value < 0.01 were regarded as candidates for further investigation.

    4c3880bb027f159e801041b1021e88e8 Result
    

    Brain metastasis showed upregulations of MCOLN3 and YTHDF2 (unadjusted P-value= 0.0093 and 0.0063, respectively) compared to the primary site. Conversely, primary site showed upregulations of IFNAR1, TNFRSF4, CXCL11, CT45A1, MAP3K5, TAL1, LAG3 and MARCO.

    In LATE-BREAKING ABSTRACT, we will report following data:

    1) Immunohistochemistry results of the candidate proteins in resected brain metastasis and primary sites of NSCLC from larger population. In situ hybridization is also planned.

    2) Additional data of expression profiles of the candidate genes in sphere cell line from brain metastasis and primary site of NSCLC.

    

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    Biologic profilings of brain metastasis from NSCLC may subsequently help to understand underlying mechanism and ultimately lead to novel targeted therapy.

    Futher details will be added in LATE-BREAKING ABSTRACT.

    6f8b794f3246b0c1e1780bb4d4d5dc53

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