Virtual Library

Start Your Search

Antonio José Maria Cataneo



Author of

  • +

    P3.12 - Small Cell Lung Cancer/NET (Not CME Accredited Session) (ID 978)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall
    • +

      P3.12-07 - MicroRNA Deregulation in a Typical Carcinoid Tumor: Potential Role of Immune Response and Invasion in Tumorigenesis.  (ID 13023)

      12:00 - 13:30  |  Author(s): Antonio José Maria Cataneo

      • Abstract
      • Slides

      Background

      Lung carcinoid tumors comprise an uncommon type of neuroendocrine cancer, and are classified as typical or atypical according to their histological characteristics. Typical carcinoid tumors are more common and usually not associated with metastasis at diagnosis. To date, genetic and epigenetic changes, as well as regulatory mechanisms mediated by non-coding RNAs and their association with the development and progression of lung carcinoid tumors are not understood. Considering that microRNAs (miRNAs) are potent gene expression regulators associated with several cancer types, our goal was to determine global miRNA expression changes in a rare case of typical lung carcinoid tumor and its corresponding metastasis from the same patient.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      RNA was isolated from two fragments of the same tumor and its paired lymph node metastasis, using the RecoverAll Total Nucleic Acid Isolation Kit for FFPE tissues (Ambion/Thermo Fisher). miRNA profiling was performed using the TaqMan Array Human microRNA card A v.3.0 platform (Life Technologies/Thermo Fisher). miRNA expression was analyzed in the Expression Suite software using the global normalization algorithm provided. Furthermore, we applied bioinformatic methods for prediction of miRNA target genes, using microRNA data integration portal, mirDIP, and to determine validated targets in lung tissue as well as molecular pathways, using miRTarBase and ToppGene Suite.

      4c3880bb027f159e801041b1021e88e8 Result

      16 miRNAs were commonly deregulated in the two fragments of tumor and the corresponding lymph node metastasis from the same patient. Of these, 15 miRNAs were significantly down-regulated (FC>=2 and p<0.01). Pathways identified were enriched for miRNA target genes associated with immune response, invasion and metastasis. Notably, many of the identified miRNAs, which were consistently down-regulated, are known to have a suppressor role in tumorigenesis.

      8eea62084ca7e541d918e823422bd82e Conclusion

      Down-regulated miRNAs may be associated with metastatic progression of typical carcinoid tumors, since a subset of 15 miRNAs were down-regulated in paired primary tumor and metastasis samples. Our results contribute to improve our understanding of miRNA regulation and disease pathogenesis in lung carcinoid tumors.

      6f8b794f3246b0c1e1780bb4d4d5dc53

      Only Active Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login or select "Add to Cart" and proceed to checkout.