Author of

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  P3.09 - Pathology (Not CME Accredited Session) (ID 975)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall

  • 27594

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    P3.09-17 - Molecular Profile in Lung Adenocarcinoma. A Muticenter Study in Cordoba, Argentina.   (ID 13933)

    12:00 - 13:30  |  Author(s): Maximiliano Franco Canigiani
    • Abstract
    • Slides

    Background
    

    Substantial advances have been made in the understanding of the biology of NSCLC in relation to the characterization of molecular abnormalities such as activations of oncogenes by mutations, translocations and amplifications, which are being used as molecular targets and predictive biomarkers. Molecular analysis of NSCLC, adeno carcinoma (AC) is now the standard of care for therapy selection

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    We determined the frequency of molecular alterations in EGFR and gene fusion ALK in our Caucasian and Hispanic populations to decide the adequate treatment .

    206 small biopsies and resection specimens of patients with NSCLC (AC) in different institutions of Cordoba were studiesduring a period (2014 - 2018).

    In addition to Histopathology Type, we analyzed immunohistochemistry (IHC) characteristics and molecular profiles and Several clinical variables were studied.

    Different tests were used to detect alterations of EGFR and fusion gene EML4-ALK expression, with the aim to identify our own profile and decide the adequate therapeutical option. EGFR mutation was studied by therascreen kit, PCR, in order to detect genetic alterations in exons 18, 19, 20 and 21. ALK translocations were analyzed by FISH (Vysis- Break Apart, Abbott) and IHC (clon D5F3, ventana, Roche). We correlated the molecular profile with different clinical variables (age, gender, and tobacco habits). The statistical method used was the multiple regression logistic model.

    4c3880bb027f159e801041b1021e88e8 Result
    

    146 men and 60 women out of 206 samples were tested for EGFR expression. Twenty nine men and sixteen women expressed EGFR positive. Activating kinase-domain mutations in EGFR were identified in 45 pts (21, 95 %): exon 19 deletion = 23, L858R = 7, exon 20 insertion = 11, other = 4. EGFR alterations were associated with gender (p=0.044), women showed more alterations of the genes. Age and smoking habit of patients did not show significant association (p=0.757 and p=0.547, respectively). We used the multiple regression logistic model to correlate EGFR expression to age, gender, tobacco habits. We identified 10 pts (5%) with fusion gene EML4-ALK. ALK alterations were not related to gender (p=0.449), age (p=0.837) and smoking habit (p=0.452).

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    Our results showed a comparable frequency in EGFR mutations and gene fusion ALK in relation to the data published in western population. These results allow a proper diagnosis to provide pts with the most adequate therapy.

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