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Deepak Koppaka



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    P3.03 - Biology (Not CME Accredited Session) (ID 969)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall
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      P3.03-20 - Circulating Tumor Cell Clustering in Vitro Short-Term Culture Correlates with Poor Survival and Allows Monitoring Response to Treatment (ID 13640)

      12:00 - 13:30  |  Author(s): Deepak Koppaka

      • Abstract

      Background

      Circulating tumor cells (CTCs) are putative markers for tumor prognosis and may serve to evaluate patient’s response to chemotherapy. CTCs are often detected as single cells but infrequently as clusters indicative of worse prognosis.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Blood samples were obtained from the 86 breast cancer patients, 52 lung cancer patients.

      The nucleated cells were cultured in ellipsoidal microwells

      The microwells were prepared by placing a cover slip (22mm X 22mm) on a 35mm culture dish. Molten agar (3%) was poured on the top of the cover slip enough to cover it (500µl approx). The ellipsoidal microwell in PDMS (polydimethylsiloxane) was then placed on the top of the cover slip containing the molten agar and allowed it to solidify. The culture conditions were carried at 37°C, 5% CO2 and humidified hypoxic conditions (1% O2). The culture medium was replaced every 48-72 hrs with nominal disturbances to the microwells. Cultures were maintained for 3 weeks and imaged on day 7, 14, 21 with bright field microscope and analyzed the images using (Olympus IX71).

      4c3880bb027f159e801041b1021e88e8 Result

      To correlate cluster formation with survival, samples were obtained from 31 patients with lung cancer. These patients were enrolled, when they were presented with progressive disease since their last treatment regimen but before commencing a new treatment regimen. Blood was collected before and on 9th, 12th week after the treatment. It is interesting to note that tight cluster formation correlated with patient survival. The Kaplan-Meier survival analysis showed that cluster formation in patients who have undergone neoadjuvant therapy correlated with shorter overall survival Our culture system enabled us to successfully expand the CTCs without any prior enrichment. The method requires 2.5 ml of blood per 35-mm dish to expand CTCs. Microwells were easy to establish and replicate with minimal set-up in a laboratory.

      8eea62084ca7e541d918e823422bd82e Conclusion

      To the best of our knowledge, this study is one of the few, which suggest the possible role of cluster formation in patient survival in Lung cancer patients.

      6f8b794f3246b0c1e1780bb4d4d5dc53