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Tali Feinberg



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    P3.03 - Biology (Not CME Accredited Session) (ID 969)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall
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      P3.03-15 - Lung Cancer Regulation of Glucose Metabolic Stress Response (ID 13850)

      12:00 - 13:30  |  Author(s): Tali Feinberg

      • Abstract

      Background

      Cancer cells have altered metabolism in order to accommodate for the increased metabolic needs required for oncogenic transformation, proliferation, development and survival. The metabolic profile observed in cancer cells often includes increased consumption of glucose and glutamine, increased glycolysis, changes in the use of metabolic enzyme isoforms, and increased secretion of lactate. In order to better understand this hallmark of cancer, we studied the regulation of pathways by microRNAs (miRNA) expression profile under normal or deprived glucose condition in different lung cancer cell lines.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Two different NSCLC adenocarcinoma cell lines were tested in the study, A549, which is highly glycolytic (HG) and H358, which is low glycolytic (LG). Sequencing was performed using Illumina TruSeq RNA library preparation kit v2 with Illumina HiSeq 2500 sequencer using single-end 50bp protocol. Cell were analysed in two different conditions, under 24h of normal glucose concentration and under 24h glucose starvation. Samples were prepared in duplicates from the initial step of cell culturing summarizing to a total of eight samples. Differential expression analysis was performed using EdgeR algorithm with an adjusted p-value >0.05 and a log2 fold > 1.5.

      4c3880bb027f159e801041b1021e88e8 Result

      An opposite regulation profile was observed for miRNA of cells that are highly glycolytic (HG), A549, in comparison with low glycolytic, H358 cells. The formers presented 42 downregulated miRNAs from which 39 miRNAs were unique. Only 2 upregulated miRNAs, miR324 and miR616, were found significant in this cohort but they are also upregulated in the latter group. In comparison, from the 20 upregulated miRNAs found in high-glycolygic group from which 18 were unique to lower glycolytic cell line. Lower glycolytic cell lines show 4 downregulated miRNAs from which only one miRNA, miR205 was significantly uniquely downregulated, moreover, MIR205HG, which is miR205 host gene, is also found significantly downregulated, suggesting the strong involvement of miR205 in glucose metabolic stress response.

      8eea62084ca7e541d918e823422bd82e Conclusion

      Under glucose metabolic stress, miR205 and MIR205HG are uniquely downregulated in low glycolytic NSCLC adenocarcinoma cell line.

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