Author of

• 25957

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  P3.03 - Biology (Not CME Accredited Session) (ID 969)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall

  • 27478

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    P3.03-03 - Differential Microbiota Features in Lung Tumor and Adjacent Normal Tissues in Lung Cancer Patients (ID 13547)

    12:00 - 13:30  |  Author(s): Jianzhong Hu
    • Abstract

    Background
    

    Emerging evidence has demonstrated the link between the host microbiota and varied malignancies, including colorectal, gastric, hepatocellular, and pancreatic cancers. However, for lung cancer, one of the leading cause of the cancer-related morbidity and mortality worldwide, the interplay between the lung cancer and the lung microbiome has yet not been well investigated. In this study, we surveyed and compared the microbiota composition and diversity in paired tumor and adjacent normal tissues to test whether any tumor specific microbial features can be identified in tumor tissues.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    We surveyed the microbiota composition of 55 lung tumor and 55 paired adjacent normal tissue samples using bacterial 16S sequencing protocol. The microbial diversity was further analyzed using QIIME pipeline. The differential taxa feature by tumor status was selected using LefSe method.

    4c3880bb027f159e801041b1021e88e8 Result
    

    We observed diversified microbiota in lung tissue samples. The dominant phyla include Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria. The overall microbiota similarity in paired tumor and adjacent tissues was significantly higher than unpaired (p<0.01). Compared to adjacent normal tissues, the lung tumors showed significantly lower alpha diversity (p=0.05) but no difference in beta diversity (PERMANOVA test, p-value=0.27). At taxa level, tumor samples showed increased Modestobacter and decreased Propionibacterium, and Enterobacteriaceae.

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    In conclusion, our study demonstrated and compared the lung microbiota diversity and composition in paired tumor and adjacent normal tissues. Compared to non-tumor tissues, the reduction of potential pro-inflammatory microbial families/genera in tumor tissues suggest the possible link between the tumor microbiota to the immunosuppression tumor microenvironment.

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