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Latifa Badre

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    P3.01 - Advanced NSCLC (Not CME Accredited Session) (ID 967)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall
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      P3.01-50 - Identification of EGFR Mutational Profile in Lung Cancer Moroccan Cohort (ID 12352)

      12:00 - 13:30  |  Author(s): Latifa Badre

      • Abstract
      • Slides


      Despite recent progress in diagnostic and oncology therapy, lung cancer constitutes the leading cause of cancer-associated mortality worldwide, with approximately 85% of lung cancer cases being non-small cell lung cancer (NSCLC) histological type. The study of epidermal growth factor receptor (EGFR) gene mutational profile in non-small cell lung cancer patients has a special clinical significance in the selection of patients for tyrosine-kinase inhibitor therapy. The aim of this study was to identify the frequency and spectrum of EGFR mutations in a cohort of Moroccan patients with lung cancer using the ADx-ARMS technology.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      We performed a retrospective study by processing 125 cases of NSCLC patients recruited between March 2015 and December 2017. Using the DNA extracted from the FFPE tissue, we attempted to identify somatic mutations in exons 18 to 21 of the tyrosine-kinase “TK” domain of EGFR gene. We evaluated EGFR mutations using HRM polymerase chain reaction (PCR) and real time PCR “ADx-ARMS technology” for results confirmation.

      4c3880bb027f159e801041b1021e88e8 Result

      Most of our patients were males (69.6%) in the age range of 32-82 years old, with an average age of 61,5 ± 8.62. The most represented histological type in the studied sample was adenocarcinoma (ADK: 88%) and only 12% of squamous cell carcinoma (SCC) subtype. In regards to the smoking status 57.6% of the analyzed patients were smokers and 42.4% were nonsmokers. Six different point mutations were detected in 25 patients, representing 20% of our study population. Among the mutant positive cases, the resulting mutations were as follows : 60% of patients have a deletion in exon 19, 16% in exon 20 (12% T790M and 4% S768L), 12% in exon 18 (G719A/C) and 12% in exon 21(L858R). The EGFR mutations were more frequent among males compared to females (64,0% and 36,0% respectively), all of the positive patients with EGFR mutations were ADK and 40,0% of them were smokers.

      8eea62084ca7e541d918e823422bd82e Conclusion

      The presented method can be implemented at the laboratories to identify the most frequent EGFR mutations that are important for targeted therapy of advanced lung cancer patients.


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