Author of

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  P3.01 - Advanced NSCLC (Not CME Accredited Session) (ID 967)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall

  • 27392

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    P3.01-38 - Therapeutic Effects of FMNP-MSC for Hyperthermia on Lung Cancer A549 in Nude Mice (ID 13302)

    12:00 - 13:30  |  Presenting Author(s): Runlei Hu
    • Abstract
    • Slides

    Background
    

    To study therapeutic effects of fluorescent magnetic nanoparticles labeled mesenchymal stem cells (FMNP-MSC) for hyperthermia induced by an alternating magnetic field on lung cancer A549 in nude mice.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    The fluorescent magnetic nanoparticles (FMNP) was prepared and characterized, and then were used to label mouse mesenchymal stem cells（MSCs) in vitro. The FMNPs-labeled MSCs were injected via tail vein into nude mice bearing non-small cell lung cancer A549 cells. The subcutaneous lung cancer tissues in the nude mice models were subsequently treated in external alternating magnetic field once a week for four weeks, temperature of tumor was detected by optical fiber. Four weeks after treatment, the tumor were collected after euthanizing the animals. Pathology change of tumor tissue was detected by light microscope, cell apoptosis proteins (Bax, Caspase-3) and anti-apoptotic protein (Bcl-2) were detected by Western blot

    4c3880bb027f159e801041b1021e88e8 Result
    

    In vivo experiments confirmed that MSC FMNP can be carried to the tumor area. Temperature of tumor can heat up to 53 ℃ under alternating magnetic field. FNMP - MSC thermal therapy group mice tumor volume and weight growth was significantly inhibited, FMNP-MSC group of mice with tumor volume growth is restrained, the weight was not significantly inhibited. Apoptosis and necrosis change of tumor tissue could be detected by light microscope. Western blot confirmed, Bax protein expression in two treatment group obviously, and Caspase 3, the Bcl-2 in the two treatment groups have no significant expression

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    FMNP-MSC can accurately targeting to the tumor area, which heat up to the effective temperature under alternating magnetic field, the growth of tumor in mice was significantly inhibited; apoptosis and necrosis could be seen in tumor, may be achieved by increasing the expression of Bax

    6f8b794f3246b0c1e1780bb4d4d5dc53

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