Virtual Library

Start Your Search

Dragana Jovanovic



Author of

  • +

    P2.16 - Treatment of Early Stage/Localized Disease (Not CME Accredited Session) (ID 965)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
    • +

      P2.16-05 - Hypermethylation of SOX1, RASSF1A, HOXA9, CDH13 and DAPK Genes Plays a Role in NSCLC Pathogenesis (ID 12376)

      16:45 - 18:00  |  Author(s): Dragana Jovanovic

      • Abstract
      • Slides

      Background

      A number of genes have been reported as aberrantly methylated in lung tumors. Here, we investigate the relationship between gene methylation in lung tumors relative to matching normal lung tissue, and whether DNA methylation changes can be detected in paired blood samples.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Primary tumor samples (n=65), corresponding nonmalignant lung tissues (n=65) and maching blood samples (n=51) were obtained from NSCLC patients undergoing curative resectional surgery. Using bisulfite pyrosequencing, CpG methylation was quantified at seven genes (RASSF1A, CDH13, MGMT, ESR1, HOXA9, SOX1 and DAPK) in lung tumor, matching pathologically normal lung tissue, and circulating blood samples. We wanted to determine whether these methylation changes are specific to lung tumors, and test whether these changes are detectable in patients’ blood samples. We also analyzed possible associations between DNA methylation and clinicopathologic features.

      4c3880bb027f159e801041b1021e88e8 Result

      We observed that genes SOX1, RASSF1A, HOXA9, CDH13 and DAPK were significantly hypermethylated in lung tumors compared to match normal tissue. However, these changes could not be detected in patients’ blood samples, indicating a low feasibility of detecting lung cancer by analyzing these genes in a blood-based test. We confirmed that hypermethylation of SOX1, RASSF1A, HOXA9, CDH13 and DAPK play a role in NSCLC pathogenesis, but also showed that these genes are not suitable markers for early detection of NSCLC. Lastly, we found that histology and gender were associated with methylation at the CDH13 gene, while stage was associated with methylation at MGMT.

      image 1.jpg

      8eea62084ca7e541d918e823422bd82e Conclusion

      Our results show higher methylation of SOX1, RASSF1A, HOXA9, CDH13 and DAPK genes in lung tumors compared to matching normal lung tissue. The lack of reflection of these methylation changes in blood samples from patients with NSCLC indicates their poor suitability for a screening test.

      6f8b794f3246b0c1e1780bb4d4d5dc53

      Only Active Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login or select "Add to Cart" and proceed to checkout.