Author of

• 25948

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  P2.11 - Screening and Early Detection (Not CME Accredited Session) (ID 960)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall

  • 27139

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    P2.11-17 - MicroRNAs in Exhaled Breath Condensate and Bronchial Brushings as Biomarkers for Early Diagnosis of Lung Cancer (ID 13124)

    16:45 - 18:00  |  Author(s): Samarpana Chakraborty
    • Abstract

    Background
    

    Lung cancer is one of the primary causes of death worldwide. According to Global Burden of Disease (GBD), highest prevalence and mortality of NSCLC is found in men in India. These typically may present as nodules or masses in the lung. The circulating microRNAs delineate stable and reproducible markers for lung cancer and may play important role in non-invasive diagnostic marker. We have previously discovered miRNA signatures in the exhaled breath condensate (EBC) that are suitable for early diagnostics of various lung diseases. Since miRNAs have roles in the pathogenesis of lung diseases, they can be potentially useful in the early diagnosis of lung cancer and may as well complement the other conventional investigative methods such as low dose computerized tomography (LDCT) to avoid high false positive rates.

    Due to paucity of thoracic surgeons and interventional bronchoscopists in India, the inaccessibility of the suspected tumor tissue for pathological diagnosis is a big challenge. All India Institute of Medical Sciences, New Delhi is one of the pioneer institutions that provides medical care to patients coming from different ethnic and socioeconomic backgrounds in India. Based on our previous work and expertise in this area along with emerging consensus in the field, this work is expected to yield promising outcomes.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    Exhaled Breath Condensate Collection:

    Samples were collected from the suspected NSCLC patients during OPD by using RTube.

    miRNA isolation from

    c-DNA synthesis using Quantimir qPCR kit

    qPCR reaction setup for profiling

    Bronchoscopy procedure:

    Bronchial brushings were collected after obtaining written informed consent during routine diagnosis.

    miRNA isolation

    c-DNA synthesis by using Kit from Applied Biosystems

    qPCR reaction setup by using Kapafast SybrGreen

    4c3880bb027f159e801041b1021e88e8 Result
    

    In this study, we found the pro tumorigenic miRNAs like miR-19a, miR-33b, miR-558 are found to be upregulated and anti-tumor miRNAs like let7b, miR-132 and miR-181c found to be downregulated in the exhaled breath of NSCLC patients. Further we validated these miRNAs in bronchial brushings samples taken from respected patients. Surprisingly; those miRNAs showed positive trend.

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    In this study, we have found various differentially expressed MicroRNAs in the exhaled breath as well as bronchial brushings samples taken from diagnosed NSCLC patients. The known pro tumorigenic miRNAs were found to be upregulated and anti tumorigenic miRNAs were downregulated. Hence, these biomarkers could either be used as a preliminary non-invasive screening method for the diagnosis of NSCLC. Apart from this, these identified miRNAs could be novel therapeutic targets for the treatment of NSCLC.

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