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Sellama Nadifi



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    P2.11 - Screening and Early Detection (Not CME Accredited Session) (ID 960)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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      P2.11-11 - Identification of the Genetic Determinants of Susceptibility to Lung Cancer in a Moroccan Cohort (ID 12508)

      16:45 - 18:00  |  Author(s): Sellama Nadifi

      • Abstract
      • Slides

      Background

      Lung cancer is the most common cause of death from cancer worldwide, estimated to be responsible 1.59 million deaths, representing 19.4% of all cancer types. Representing 80–85% of all cases non-small cell lung cancer (NSCLC) is the most predominant type of lung cancer. Lung cancer has been recognized as a complex multifactorial disease resulting from the interactions between various genetic and environmental factors. Identification of genes involved in the occurrence and development of lung cancer could contribute to further understanding of the underlying mechanisms, and even a very important additional appropriate prevention strategies and targeted treatments for reducing lung cancer burden. Many studies have suggested that key cytokines in inflammation pathways may exert important roles in the etiology of lung cancer. The aim of this study was to investigate whether common variants in inflammatory and immune response genes influence lung cancer risk in Moroccan patients.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Using a candidate gene approach, 9 single nucleotide polymorphisms (SNPs) in 5 genes were assessed in 308 controls and 120 lung cancer patients. Genotyping was performed with the TaqMan® allelic discrimination technology. The results were analysed using SPSS 24.0 software.

      4c3880bb027f159e801041b1021e88e8 Result

      Among the 5 studied genes, we found a significant association for the IL17A ( rs7747909) polymorphisms, IL-6_rs2069840a trend of association was observed for without reaching the significance level. No significant association was observed for the remaining SNPs in the following genes: MIF_rs755622, IL-6R_rs2228145, IL6ST_rs2228044, TYK2_(rs2304856, rs34536443, rs35018800), STAT3 rs2293152.

      8eea62084ca7e541d918e823422bd82e Conclusion

      The results founded suggest the important role of genetically determined high inflammatory response in the pathogenesis of lung cancer in the Moroccan population.

      6f8b794f3246b0c1e1780bb4d4d5dc53

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    P3.01 - Advanced NSCLC (Not CME Accredited Session) (ID 967)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/26/2018, 12:00 - 13:30, Exhibit Hall
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      P3.01-50 - Identification of EGFR Mutational Profile in Lung Cancer Moroccan Cohort (ID 12352)

      12:00 - 13:30  |  Author(s): Sellama Nadifi

      • Abstract
      • Slides

      Background

      Despite recent progress in diagnostic and oncology therapy, lung cancer constitutes the leading cause of cancer-associated mortality worldwide, with approximately 85% of lung cancer cases being non-small cell lung cancer (NSCLC) histological type. The study of epidermal growth factor receptor (EGFR) gene mutational profile in non-small cell lung cancer patients has a special clinical significance in the selection of patients for tyrosine-kinase inhibitor therapy. The aim of this study was to identify the frequency and spectrum of EGFR mutations in a cohort of Moroccan patients with lung cancer using the ADx-ARMS technology.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      We performed a retrospective study by processing 125 cases of NSCLC patients recruited between March 2015 and December 2017. Using the DNA extracted from the FFPE tissue, we attempted to identify somatic mutations in exons 18 to 21 of the tyrosine-kinase “TK” domain of EGFR gene. We evaluated EGFR mutations using HRM polymerase chain reaction (PCR) and real time PCR “ADx-ARMS technology” for results confirmation.

      4c3880bb027f159e801041b1021e88e8 Result

      Most of our patients were males (69.6%) in the age range of 32-82 years old, with an average age of 61,5 ± 8.62. The most represented histological type in the studied sample was adenocarcinoma (ADK: 88%) and only 12% of squamous cell carcinoma (SCC) subtype. In regards to the smoking status 57.6% of the analyzed patients were smokers and 42.4% were nonsmokers. Six different point mutations were detected in 25 patients, representing 20% of our study population. Among the mutant positive cases, the resulting mutations were as follows : 60% of patients have a deletion in exon 19, 16% in exon 20 (12% T790M and 4% S768L), 12% in exon 18 (G719A/C) and 12% in exon 21(L858R). The EGFR mutations were more frequent among males compared to females (64,0% and 36,0% respectively), all of the positive patients with EGFR mutations were ADK and 40,0% of them were smokers.

      8eea62084ca7e541d918e823422bd82e Conclusion

      The presented method can be implemented at the laboratories to identify the most frequent EGFR mutations that are important for targeted therapy of advanced lung cancer patients.

      6f8b794f3246b0c1e1780bb4d4d5dc53

      Only Active Members that have purchased this event or have registered via an access code will be able to view this content. To view this presentation, please login or select "Add to Cart" and proceed to checkout.