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Anshuman Chaturvedi



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    P2.09 - Pathology (Not CME Accredited Session) (ID 958)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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      P2.09-06 - Expression of PD-L1 on Routine Non-Small Cell Lung Carcinoma Sections: Comparative Assessment of SP263 (Ventana) and 22C3 (DAKO pharmDx) (ID 13019)

      16:45 - 18:00  |  Author(s): Anshuman Chaturvedi

      • Abstract
      • Slides

      Background

      The SP263 (Ventana Benchmark) antibody as a predictive immunohistochemical marker for pembrolizumab therapy provides an avenue for local testing. Pathologists without access to the Dako Autostainer Link 48 platform (certified for the Dako 22C3 antibody) have been restricted to referrals at external departments, resulting in an increased turnaround time. Here we report the results of our local verification of SP263.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Specimens previously assessed for 22C3 PD-L1 expression at either Royal Liverpool Hospital or Queen Elizabeth Hospital Birmingham were selected from the archives of Wythenshawe Hospital. Cases with less than 100 viable residual tumour cells were excluded. The same tumour block was selected for staining with the Roche SP263 clone and specimens were assessed for tumour proportion score (TPS), immune cell proportion and staining intensity. Assays were reported as disagreeing if a differing TPS changed the therapeutic cut-off ranges.

      4c3880bb027f159e801041b1021e88e8 Result

      Expression levels of 22C3 and SP263 were compared across 100 cases (43 resections, 26 biopsies, 26 lymph node aspirates, 5 node excisions); 59 adenocarcinomas, 33 squamous carcinomas, 8 not otherwise specified (70 primary, 30 metastatic). The TPS ranges (<1%, 1 - 49%, > 50%) were in agreement for 78 samples. Of the 22 cases with differing ranges, 15 reflected a TPS of <10% and 7 had greater differences e.g. 10% versus 60%. Reasons for discrepancies included faint membranous staining on a few of the 22C3 sections (not apparent on SP263), scoring of carcinoma in situ, possible scoring of cells at a deeper block level, and variation in interpretation by the scoring pathologists. The overall Pearson correlation coefficient (r) was 0.9025, p < 0.00001.

      Table 1 Comparison of PD-L1 Ventana SP263 and Dako 22C3 tumour proportion scores
      SAMPLE DIAGNOSIS SP263 >/= 1% >/= 50%

      Liverpool

      22C3

      Birmingham

      22C3

      Discrepancy

      Pleural biopsy

      Adenocarcinoma

      1

      No

      No

      1

      N/A

      No

      Pleural biopsy

      Adenocarcinoma

      10

      No

      No

      60

      N/A

      Yes

      EBUS node

      Metastatic adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      EBUS node

      Metastatic adenocarcinoma

      0

      No

      No

      1

      N/A

      Yes

      Resection

      Adenocarcinoma

      40

      Yes

      No

      10

      N/A

      No

      EBUS node

      Metastatic squamous carcinoma

      2

      No

      No

      10

      N/A

      No

      Resection

      Adenocarcinoma

      5

      Yes

      No

      <1

      N/A

      Yes

      EBUS node

      Metastatic adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      Bronchial biopsy

      Squamous carcinoma

      10

      Yes

      No

      70

      N/A

      Yes

      Bronchial biopsy

      Squamous carcinoma

      0

      No

      No

      0

      N/A

      No

      EBUS node

      Metastatic squamous carcinoma

      <1

      No

      No

      <1

      N/A

      No

      EBUS node

      Metastatic adenocarcinoma

      <1

      No

      No

      0

      N/A

      No

      Bronchial biopsy

      Squamous carcinoma

      40

      Yes

      No

      40

      N/A

      No

      Resection

      Squamous carcinoma

      5

      Yes

      No

      10

      N/A

      No

      Lung biopsy

      Adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      Lymph node biopsy

      Metastatic adenocarcinoma

      100

      Yes

      Yes

      100

      N/A

      No

      Resection

      Adenocarcinoma

      <1

      No

      No

      N/A

      <1

      No

      Lung biopsy

      Adenocarcinoma

      0

      No

      No

      N/A

      0

      No

      Resection

      Adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      Resection

      Adenocarcinoma

      10

      Yes

      No

      N/A

      5 to 10

      No

      Resection

      Adenocarcinoma

      20

      Yes

      Yes

      N/A

      20-30

      No

      Resection

      Squamous carcinoma

      2 to 4

      Yes

      No

      N/A

      3 to 5

      No

      Bronchial biopsy

      Squamous carcinoma

      <1

      No

      No

      N/A

      <1

      No

      Bronchial biopsy

      Squamous carcinoma

      2 to 4

      Yes

      No

      1

      N/A

      No

      Lymph node biopsy

      Metastatic adenocarcinoma

      100

      Yes

      Yes

      100

      N/A

      No

      Bronchial biopsy

      NOS

      <1

      No

      No

      N/A

      <1

      No

      Bronchial biopsy

      Adenocarcinoma

      0

      No

      No

      1

      N/A

      Yes

      Resection

      Adenocarcinoma

      2 to 4

      Yes

      No

      10

      N/A

      No

      Lymph node biopsy

      Metastatic squamous carcinoma

      80

      Yes

      Yes

      70

      N/A

      No

      EBUS node

      Metastatic adenocarcinoma

      80

      Yes

      Yes

      80

      N/A

      No

      Bronchial biopsy

      Squamous carcinoma

      0

      No

      No

      N/A

      <1

      No

      EBUS node

      Metastatic adenocarcinoma

      90

      Yes

      Yes

      80

      N/A

      No

      Pleural biopsy

      Adenocarcinoma

      0

      No

      No

      10

      N/A

      Yes

      Bronchial biopsy

      Squamous carcinoma

      0

      No

      No

      0

      N/A

      No

      Bronchial biopsy

      Squamous carcinoma

      5

      Yes

      No

      N/A

      0

      Yes

      EBUS node

      Metastatic squamous carcinoma

      50

      Yes

      Yes

      50

      N/A

      No

      Bronchial biopsy

      Squamous carcinoma

      2 to 4

      Yes

      No

      10

      N/A

      No

      EBUS node

      Metastatic adenocarcinoma

      70

      Yes

      Yes

      N/A

      70

      No

      EBUS node

      Metastatic adenocarcinoma

      100

      Yes

      Yes

      100

      N/A

      No

      Bronchial biopsy

      Squamous carcinoma

      1

      Yes

      No

      20

      N/A

      No

      Resection

      Adenocarcinoma

      5

      Yes

      No

      0

      N/A

      Yes

      Resection

      Adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      EBUS node

      Adenocarcinoma

      <1

      No

      No

      0

      N/A

      No

      Resection

      Adenocarcinoma

      5

      Yes

      No

      10

      N/A

      No

      EBUS node

      Sarcomatoid carcinoma

      0

      No

      No

      0

      N/A

      No

      Resection

      Adenocarcinoma

      0

      No

      No

      N/A

      <1

      No

      Lymph node biopsy

      Metastatic squamous carcinoma

      0

      No

      No

      N/A

      <1

      No

      Resection

      Adenocarcinoma

      60

      Yes

      Yes

      N/A

      60-70

      No

      Bronchial biopsy

      Squamous carcinoma

      0

      No

      No

      0

      N/A

      No

      Pleural biopsy

      Adenocarcinoma

      2 to 4

      Yes

      No

      10

      N/A

      No

      Lung biopsy

      Squamous carcinoma

      0

      No

      No

      5

      N/A

      Yes

      EBUS node

      Metastatic adenocarcinoma

      5

      Yes

      No

      30

      N/A

      No

      Resection

      Pleomorphic carcinoma

      70

      Yes

      Yes

      80

      N/A

      No

      Resection

      Squamous carcinoma

      0

      No

      No

      1

      N/A

      Yes

      Bronchial biopsy

      Squamous carcinoma

      10

      Yes

      No

      20

      N/A

      No

      Resection

      Adenocarcinoma

      50

      Yes

      Yes

      N/A

      10 to 20

      Yes

      Resection

      Adenocarcinoma

      0

      No

      No

      N/A

      <1

      No

      Resection

      Pleomorphic carcinoma

      1

      Yes

      No

      20

      N/A

      No

      Resection

      Adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      Resection

      Adenocarcinoma

      10

      Yes

      No

      N/A

      2 to 3

      No

      EBUS node

      Metastatic adenocarcinoma

      100

      Yes

      Yes

      100

      N/A

      No

      Resection

      Adenocarcinoma

      5

      Yes

      No

      N/A

      <1

      Yes

      EBUS node

      Metastatic adenocarcinoma

      100

      Yes

      Yes

      100

      N/A

      No

      EBUS node

      Metastatic squamous carcinoma

      50

      Yes

      Yes

      N/A

      80

      No

      Resection

      Adenocarcinoma

      90

      Yes

      Yes

      95

      N/A

      No

      Resection

      Adenocarcinoma

      <1

      No

      No

      N/A

      <1

      No

      Resection

      Adenocarcinoma

      0

      No

      No

      1

      0

      Yes

      Resection

      Adenocarcinoma

      <1

      No

      No

      5

      N/A

      Yes

      Resection

      Adenocarcinoma

      20

      Yes

      No

      20

      N/A

      No

      Bronchial biopsy

      NOS

      50

      Yes

      Yes

      60

      N/A

      No

      Resection

      Adenocarcinoma

      2 to 4

      Yes

      No

      N/A

      5 to 10

      No

      Resection

      Adenocarcinoma

      0

      No

      No

      10

      N/A

      Yes

      Soft tissue

      NOS

      1

      Yes

      No

      20

      N/A

      No

      Bronchial biopsy

      Sarcomatoid carcinoma

      <1

      No

      No

      N/A

      <1

      No

      EBUS node

      Metastatic adenocarcinoma

      70

      Yes

      Yes

      N/A

      60 to 70

      No

      Pleural biopsy

      Adenocarcinoma

      80

      Yes

      Yes

      N/A

      70

      No

      Resection

      Squamous carcinoma

      <1

      No

      No

      1

      0

      Yes

      Resection

      Adenocarcinoma

      50

      Yes

      Yes

      N/A

      5 to 10

      Yes

      Resection

      Adenocarcinoma

      10

      Yes

      No

      50

      N/A

      Yes

      Resection

      Adenocarcinoma

      0

      No

      No

      0

      N/A

      No

      Resection

      Squamous carcinoma

      1

      Yes

      No

      N/A

      <1

      Yes

      Resection

      Squamous carcinoma

      100

      Yes

      Yes

      100

      N/A

      No

      Resection

      Squamous carcinoma

      5

      Yes

      No

      10

      No

      Resection

      Squamous carcinoma

      70

      Yes

      Yes

      N/A

      70 to 80

      No

      Resection

      Squamous carcinoma

      90

      Yes

      Yes

      N/A

      10 to 20

      Yes

      EBUS node

      Squamous carcinoma

      <1

      No

      No

      N/A

      <1

      No

      EBUS node

      Adenocarcinoma

      0

      No

      No

      N/A

      0

      No

      EBUS node

      Metastatic adenocarcinoma

      70

      Yes

      Yes

      N/A

      80

      No

      EBUS node

      Metastatic squamous carcinoma

      50

      Yes

      No

      N/A

      70

      No

      Resection

      Adenocarcinoma

      <1

      No

      No

      N/A

      <1

      No

      EBUS node

      Metastatic adenocarcinoma

      40

      Yes

      No

      30

      N/A

      No

      Resection

      Squamous carcinoma

      90

      Yes

      Yes

      N/A

      100

      No

      Resection

      Adenocarcinoma

      40

      Yes

      No

      40

      N/A

      No

      EBUS node

      Metastatic adenocarcinoma

      80

      Yes

      Yes

      N/A

      70 to 80

      No

      EBUS node

      Metastatic squamous carcinoma

      1

      Yes

      No

      N/A

      <1

      Yes

      Bronchial biopsy

      Squamous carcinoma

      1

      Yes

      No

      1

      N/A

      No

      EBUS node

      Metastatic adenocarcinoma

      5

      Yes

      No

      60

      N/A

      Yes

      Primary EBUS

      Pleomorphic carcinoma

      100

      Yes

      Yes

      90

      N/A

      No

      Resection

      Adenocarcinoma

      90

      Yes

      Yes

      90

      N/A

      No

      Lymph node biopsy

      Adenocarcinoma

      <1

      No

      No

      <1

      N/A

      No

      8eea62084ca7e541d918e823422bd82e Conclusion

      Tumour expression profiles of PD-L1 are similar for the 22C3 and SP263 antibodies, with a rate of variation similar to previous reports. Cases that are discrepant may reflect differences in pathologist interpretation rather than the assay.

      6f8b794f3246b0c1e1780bb4d4d5dc53

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