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Trynda Kroneman
Author of
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P2.04 - Immunooncology (Not CME Accredited Session) (ID 953)
- Event: WCLC 2018
- Type: Poster Viewing in the Exhibit Hall
- Track:
- Presentations: 1
- Moderators:
- Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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P2.04-06 - Increased Plasma Cell % and Decreased B-Cells in Tumor Immune Infiltrates Are Associated with Worse Prognosis in Lung Adenocarcinomas (ID 12303)
16:45 - 18:00 | Author(s): Trynda Kroneman
- Abstract
Background
Clinical significance of tumor-infiltrating plasma cells and B-cells in lung adenocarcinoma is not well known.
a9ded1e5ce5d75814730bb4caaf49419 Method
CD3, CD20 and MUM1 immunostains were performed on representative tumor blocks selected from 120 consecutive lung adenocarcinoma cases resected. CD3-positive T-cells, CD20-positive B-cells, and MUM1-positive plasma cells were separately enumerated in the intraepithelial (IE) compartment and the stroma (ST) by digital image analyses. Distribution of measured tumor-infiltrating cells was systematically evaluated and their associations with patient’s overall survival (OS) modeled using Cox proportional hazards analysis.
4c3880bb027f159e801041b1021e88e8 Result
Median age of patients was 69 years (range, 46-91 years) and 52 patients were male. Eighty-two, 17, and 21 patients were tumor stage I, II, and III/IV, respectively. Ninety patients had surgery only; 30 had surgery with adjuvant chemotherapy and/or radiation therapy. Median numbers (interquartile range) of CD20-positive B-cells per 1mm2 in the tumor area (IE plus ST) and in IE compartment were 590 (224-1276) and 101 (38-109), respectively; the corresponding numbers of MUM1-positive plasma cells were 298 (180-605), and 67 (22-145), respectively. The percent of MUM1-positive plasma cells among all tumor immune infiltrate (i.e. MUM1-positive cells/[CD3-positive cells + CD20-positive cells + MUM1- positive cells] x 100) ranged from 0 to 60% (median 10%) in the tumor area and showed a significant association with OS by univariate Cox analysis (continuous variable; negative correlation with HR=12.50 [95% confidence interval [CI], 1.75-89.27]). There was a significant association between IE CD20-positive B-cells and the patient’s OS in univariate analysis (continuous variable; positive correlation with hazard ratio [HR]=0.81 (95% CI, 0.68-0.96). Both parameters remained significant by multivariate analysis. Cut-off points (low vs high) showing significant associations with patient’s OS were found by log-rank test (Table 1).
8eea62084ca7e541d918e823422bd82e ConclusionTable 1. Summary of Cox proportional hazards analysis for overall survival
CD20 cells, intraepithelial
MUM1/(CD3+CD20+MUM1),
intraepithelial and stromal
Cut-offs (number)
HR (95% CI)
p
Cut-offs
(%)
HR (95% CI)
p
>72.13
0.63(0.37-0.04)
0.070
>20.4%
1.65(0.98-2.79)
0.063
<72.13
--
<20.4%
>75.49
0.59(0.36-0.99)
0.045
>21.1%
1.71(1.01-2.90)
0.047
<75.49
--
<21.1%
--
>85.66
0.49 (0.29,0.83)
0.007
>24.79%
2.29 (1.33,3.94)
0.003
<85.66
--
<24.79%
--
>101.55
0.57(0.34,0.96)
0.034
>26.0%
2.00(1.07-3.75)
0.031
<101.55
--
<26.0%
--
>101.80
0.65(0.39,1.09)
0.105
>26.4%
1.83(0.97-3.45)
0.064
<101.80
--
<26.4%
--
High plasma cell % among immune infiltrate in the tumor area and low IE B-cell count were associated with worse prognosis in lung adenocarcinoma patients.
6f8b794f3246b0c1e1780bb4d4d5dc53