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  P2.03 - Biology (Not CME Accredited Session) (ID 952)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall

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    P2.03-33 - Anti-Aging Gene, Klotho is a Predictive Factor of Pemetrexed for Lung Cancer Treatment (ID 12714)

    16:45 - 18:00  |  Presenting Author(s): Kyoshiro Takegahara
    • Abstract
    • Slides

    Background
    

    Adjuvant chemotherapy of lung cancer including cisplatin is recommended for patients with lung cancer p-stage IB-IIIA, who were received surgical resection completely. In order to improve the prognosis of the patients with lung cancers, we need to find a new predictive factor for anti-cancer agents and establish a new adjuvant chemotherapy.

    We reported that anti-aging gene Klotho expression was a prognostic factor for small cell lung cancer and LCNEC so far. In this study, we evaluated that the expression of Klotho was a prognostic factor and predictive factor for lung cancer treatment.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    We established a cell line, A549/Klotho, which stably overexpress Klotho. Next, we checked expression of Epithelial-Mesenchymal Transition related protein such as N-cadherin, E-cadherin, Snail, Vimentin, etc, by Western blot analysis. Finally, We examined the sensitivity test for various anticancer agents including pemetrexed, CDDP, paclitaxel, gefitinib, etc, using A549 cells and A549/Klotho cells.

    4c3880bb027f159e801041b1021e88e8 Result
    

    In Western blot analysis, no expression of N-cadherin was observed. This result indicated that overexpressing Klotho inhibit the expression of N-cadherin, and suggest that Klotho can regulate the EMT. Secondly, we performed sensitivity test by MTT assay. A549/Klotho cells were more sensitive seven times against pemetrexed compared to A549 cells (IC50; 0.1 micro M). There is no difference of sensitivity between A549/Klotho cells and A549 cells against molecular target drugs such as gefitinib.

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    From these result, we conclude that overexpression of Klotho may regulate the sensitivity against pemetrexed through the inhibition of N-cadherin. In the future, we may establish a new strategy of adjuvant chemotherapy for lung cancer based on the expression of anti-aging gene Klotho.

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