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Zhe Sun



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    P2.03 - Biology (Not CME Accredited Session) (ID 952)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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      P2.03-23 - MicroRNA-30e Inhibits Cell Proliferation and Invasion in Non-Small Cell Lung Cancer via Directly Targeting SOX9 (ID 12848)

      16:45 - 18:00  |  Author(s): Zhe Sun

      • Abstract
      • Slides

      Background

      Previous studies reported that microRNA-30e (miR-30e) was dysregulation in multiple human cancers. However, the expression, functions and molecular mechanism of miR-30e in NSCLC remains unknown. In this study, we found that miR-30e expressed at low levels in NSCLC tissues and NSCLC cell lines.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      figure.jpgThe miR-30e expression was texted in NSCLC tissues and cell lines by RT-qPCR. We then overexpressed miR-30e expression in NSCLC cells to evaluate its effects in regulating NSCLC proliferation and invasion in vitro. Moreover, the mechanisms underlying the biological roles of miR-30e in NSCLC were investigated using luciferase reporter assay.

      4c3880bb027f159e801041b1021e88e8 Result

      In NSCLC cell lines, enforced expression of miR-30e inhibited cell proliferation and invasion in vitro. In addition, miR-30e negatively regulated SOX9 expression through directly binding to the 3'UTR of SOX9, and an inverse correlation was found between miR-30e and SOX9 mRNA expression level in NSCLC tissues. Moreover, SOX9 knockdown led to decreased proliferation and invasion of NSCLC cells.

      8eea62084ca7e541d918e823422bd82e Conclusion

      miR-30e is downregulated in NSCLC and inhibits cell proliferation and invasion, possibly by directly targeting SOX9.

      6f8b794f3246b0c1e1780bb4d4d5dc53

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