Author of

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  P2.01 - Advanced NSCLC (Not CME Accredited Session) (ID 950)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall

  • 26909

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    P2.01-104 - Plasma T-Cell-Derived Circulating DNA in Advanced NSCLC is Not Correlated with TIL but has a Potential of Prognostic Value (ID 13771)

    16:45 - 18:00  |  Author(s): Poonchavist Chantranuwatana
    • Abstract

    Background
    

    Non-tumor derived circulating DNA (nt-cirDNA) of advanced non-small cell lung cancer (NSCLC) patient, even not yet clear originated, was associated with prognosis. In this study, we investigated whether T-cell-derived circulating DNA (T-cirDNA) was the majority part of nt-cirDNA nor correlated with tumor-infiltrating T-lymphocyte (T-TIL).

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    Prognostic impact including demographic characteristics were integrated into the model. Using Quantitative real-time PCR with Taqman assay specific to VDJ segment of TCR^β (T-cell-receptor beta chain) was used to represented amount of T-cirDNA in plasma of 106 advanced stage NSCLC. Quantitative CD3-specific immunohistochemistry (IHC) staining from biopsy specimen, represented T-TIL, was done using Aperio ImageScope.

    4c3880bb027f159e801041b1021e88e8 Result
    

    T-cirDNA was detected in seventy-three advanced NSCLC patients with a median of 1.71 pg/ml [range 0-2260]. Forty-six patients were assessed for proportion of T-TIL per total cell with a median of 0.22 per mm² [range 0.02-2.34]. No correlation was found between T-cirDNA and T-TIL. From multivariable analysis, active smoking status was the only factor correlated with low T-cirDNA level (P<0.001). Kaplan Meier survival analysis of T-cirDNA ratio (T-cirDNA/total cirDNA) shown a trend of favor prognostic outcome for high T-cirDNA ratio (more than 0.03 %), HR 0.67 [95% CI 0.43-1.04, P=0.07]

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    plasma T-cirDNA component, even not correlated with T-TIL, revealed a trend of prognostic impact in advanced stage non-small-cell cancer patients.

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