Virtual Library
Start Your Search
Jinjin Wang
Author of
-
+
P2.01 - Advanced NSCLC (Not CME Accredited Session) (ID 950)
- Event: WCLC 2018
- Type: Poster Viewing in the Exhibit Hall
- Track:
- Presentations: 1
- Moderators:
- Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
-
+
P2.01-100 - Different Genetic Mutations Enriched in Circulating Tumor DNA Predict Different Metastatic Sites in Lung Adenocarcinoma Patients (ID 13636)
16:45 - 18:00 | Author(s): Jinjin Wang
- Abstract
Background
The mutation map of the lung adenocarcinoma is clear. However, differences of genetic mutations related to metastatic sites have not been addressed before and remain to be explored. Identification of mutation signature may help to predict metastasis.
a9ded1e5ce5d75814730bb4caaf49419 Method
We reviewed 353 ctDNA samples from lung adenocarcinoma patients with definite metastasis at our institute. Somatic mutation profiles were analyzed using hybridization capture based next-generation sequencing (NGS), which enables the simultaneous detection of single-nucleotide variants, insertions/deletions, rearrangements, and copy-number alterations of 59 genes.
4c3880bb027f159e801041b1021e88e8 Result
All the samples were divided into 2 groups based on the distance of the metastases: 165 samples was in the distal metastasis group including bone or liver metastases; 188 samples was in the proximal group, including lung, pleural or thoracic lymph node metastasis. The gene mutation number of the distal metastasis is higher than the proximal metastasis (4.92 vs 3.85, P=0.031). Gene mutations for each group are shown in the figure below. Similar to the genetic profiling of lung adenocarcinoma in COMIC database, the most frequently mutated genes were EGFR and TP53 in two groups. But the frequency mutation of NTRK1 in proximal metastasis group is three times more than that of the distal metastasis group (11/188, 5.9% vs 3/165,1.8%). And the frequency of ALK mutation in the distal metastasis group is two times more than that of the proximal metastasis group (10/165, 6.1% vs 6/188, 3.2%). Moreover, for the top 9 frequently mutant genes, there was 78% overlap in the two groups. However, the overlap with COSMIC database was 55% for distal metastasis group and 44% for the proximal metastasis.
8eea62084ca7e541d918e823422bd82e ConclusionDistant metastasis group
Proximal metastasis group
COSMIC database
gene
Mutation frequency
gene
Mutation frequency
gene
Mutation frequency
1
EGFR
70.30%
EGFR
73.94%
EGFR
31%
2
TP53
60.61%
TP53
61.17%
TP53
31%
3
KRAS
11.52%
ERBB2
9.04%
KRAS
18%
4
RB1
10.30%
KRAS
9.04%
STK11
8%
5
NF1
9.70%
RB1
7.45%
CDKN2A
7%
6
ERBB2
7.88%
NF1
7.40%
SMARCA4
6%
7
APC
6.67%
APC
6.91%
NF1
6%
8
ALK
6.06%
PIK3CA
6.38%
ATM
5%
9
ATM
6.06%
RET
5.85%
KDR
5%
10
PIK3CA
6.06%
NTRK1
5.85%
Lung adenocarcinoma patients with ALK mutation are more likely to have distant metastasis. While the patients with NTRK1 mutation are more likely to have proximal metastasis.
6f8b794f3246b0c1e1780bb4d4d5dc53