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Liyan Xu



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    P2.01 - Advanced NSCLC (Not CME Accredited Session) (ID 950)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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      P2.01-86 - Genetic Profiling of Circulating Cell-Free DNA from Cerebrospinal Fluid and Plasma in ALK-Positive Lung Cancer with Brain Metastases (ID 13189)

      16:45 - 18:00  |  Author(s): Liyan Xu

      • Abstract
      • Slides

      Background

      Brain metastases (BMs) occur in approximately 30% of patients with ALK-positive non-small-cell lung cancer (NSCLC), and in patients treated with crizotinib, central nervous system progression occurs in up to 70% of patients. Resistance mechanisms of BMs with ALK rearrangement remained unclear due to limited access to BMs lesions.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      Ten NSCLC patients with BMs carrying ALK-positive in tumors determined by Ventana anti-ALK (D5F3) immunohistochemistry assay were enrolled. Cell-free DNA (cfDNA) of cerebrospinal fluid (CSF) and plasma were tested by next-generation sequencing (NGS) with 168 genes panel.

      4c3880bb027f159e801041b1021e88e8 Result

      Out of 10 patients, three were females and seven males aged from 29 to 57 years old (median age of 34 years old). In all of cases, CSF cytology were negative. In NGS assays, In patients with ALK-positive, fusion gene positive genes were detected in 30.0% (3/10), 80.0% (8/10) and 100% (8/8) patients of CSF cfDNA, plasma and tumor, respectively; and in ALK-positive of CSF, all of them had much higher allele fractions in CSF cfDNA than the other two media.13_01.png

      8eea62084ca7e541d918e823422bd82e Conclusion

      For ALK-positive lung cancer with BMs, CSF may serve as liquid biopsy by detecting cfDNA within CSF to characterize the driver and resistant genes, dynamic genetic profiling of CSF would be an appropriate choice.

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