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Wei Chen



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    P2.01 - Advanced NSCLC (Not CME Accredited Session) (ID 950)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/25/2018, 16:45 - 18:00, Exhibit Hall
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      P2.01-17 - MALAT1-Mir-101-SOX9 Feedback Loop Modulates the Chemo-Resistance of Lung Cancer Cell to DDP via Wnt Signaling Pathway (ID 11289)

      16:45 - 18:00  |  Presenting Author(s): Wei Chen

      • Abstract
      • Slides

      Background

      Cisplatin (DDP)-based chemotherapy is a standard strategy for lung cancer, while chemo-resistance remains a major therapeutic challenge. Recent evidence highlights the crucial regulatory roles of long non-coding RNAs (lncRNA) in tumor biology. Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) has important roles in regulating the proliferation, invasion and migration of lung cancer cell.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      1. Tissue samples, cell lines and reagents

      2. RNA extraction and SYBR green quantitative PCR analysis

      3. MTT assay and BrdU incorporation assay

      4. Western blot analysis

      5. RNA immunoprecipitation

      6. Chromatin immunoprecipitation (ChIP)

      7. Luciferase reporter assay

      8. Statistical analysis

      4c3880bb027f159e801041b1021e88e8 Result

      High MALAT1 expression in lung cancer was related to poorer clinicopathologic features in this study. MALAT1 knockdown alone was sufficient to amplify DDP-induced repression of cell viability. MALAT1 knockdown could also sensitized DDP-resistant lung cancer cells (A549/DDP and H1299/DDP) to DDP. Further assays indicated that MALAT1 acted as a competing endogenous RNA to upregulate SOX9 expression by sponging miR-101 in DDP-resistant cancer cells, through Wnt signaling pathway. Moreover, SOX9 could bind to the promoter of MALAT1 to activate its transcription.

      8eea62084ca7e541d918e823422bd82e Conclusion

      Taken together, MALAT1, miR-101 and SOX9 form a feedback loop to enhance the chemo-resistance of lung cancer cell to DDP; this MALAT1-miR-101-SOX9 feedback loop plays an important role in the chemo-resistance of lung cancer cell to DDP and may serve as a potential target for cancer treatment.

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