Author of

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  P1.09 - Pathology (Not CME Accredited Session) (ID 941)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/24/2018, 16:45 - 18:00, Exhibit Hall

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    P1.09-28 - Clinical Utility of Rapid Immunohistochemistry for Differentiation of Solitary Pulmonary Adenocarcinomas (ID 12333)

    16:45 - 18:00  |  Author(s): Nobuyasu Kurihara
    • Abstract

    Background
    

    Intraoperative differentiation between pulmonary metastasis and primary lung cancer is necessary for determining the appropriate range of excision. Although conventional immunohistochemistry is often used for such differentiation, its intraoperative use is limited by time constraints. We therefore developed a device that enables complete and rapid immunohistochemistry (R-IHC) within 20 minutes, and used it with anti-thyroid transcription factor-1 (TTF-1) for differential diagnosis of pulmonary adenocarcinoma. However, the diagnostic accuracy of the method likely can be improved by using a combination of two or more antibodies. We therefore evaluated the clinical utility of R-IHC with combinations of antibodies for discriminating the etiology of solitary pulmonary adenocarcinomas.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    Twenty-two patients with pulmonary adenocarcinomas treated between February 2015 and May 2017 were enrolled in this prospective study. Tumor samples were sectioned, labeled with multiple antibodies using R-IHC, and pathologically evaluated. The standard used for comparison was conventional hematoxylin eosin (HE) staining and IHC.

    4c3880bb027f159e801041b1021e88e8 Result
    

    All intraoperative diagnoses made using R-IHC were consistent with the final diagnosis; that is, the accuracy was 100%. Moreover, the respective staining with each antibody corresponded between intraoperative R-IHC and IHC with the permanent specimen. For rapid intraoperative diagnosis differentiating pulmonary adenocarcinoma from lung metastasis from colon cancer, R-IHC with a combination of TTF-1, cytokeratin (CK) 7, and CK20 antibodies was highly effective.

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    Our novel R-IHC method with multiple antibodies was highly effective for diagnosis and differentiation of solitary pulmonary adenocarcinomas. This technology may prove to be an important supplement to standard intraoperative pathologic diagnosis in routine practice.

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