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  P1.03 - Biology (Not CME Accredited Session) (ID 935)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/24/2018, 16:45 - 18:00, Exhibit Hall

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    P1.03-31 - Periostin is a Negative Prognostic Factor and Promotes Cancer Cell Proliferation in Non-Small Cell Lung Cancer (ID 11287)

    16:45 - 18:00  |  Presenting Author(s): Toshimasa Okazaki
    • Abstract
    • Slides

    Background
    

    Periostin is a matricellular protein that is secreted by fibroblasts and interacts with various cell-surface integrin molecules. Although periostin is known to support tumor development in human malignancies, little is known about its effect on lung‑cancer progression.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    We classified 189 clinical specimens from patients with non‑small cell lung‑cancer according to high or low periostin expression. In a syngenic implantation model, Ex3LL cells (mouse lung cancer cell line, 1×106 in 100‑µL PBS) were implanted into the left thigh muscle of periostin–/– or periostin+/+ mice. Tumor formation was monitored weekly. Four weeks after implantation, the mice were then euthanized. The primary tumors and metastatic lung nodules were assessed.

    4c3880bb027f159e801041b1021e88e8 Result
    

    We found a better prognosis for patients with low rather than high periostin, even in cases of advanced‑stage cancer.　In a syngenic implantation model, murine Ex3LL lung‑cancer cells formed smaller tumor nodules in periostin−/− mice than in periostin+/+ mice, both at the primary site and at metastatic lung sites. An in vitro proliferation assay showed that stimulation with recombinant periostin increased Ex3LL-cell proliferation. We also found that periostin promotes ERK phosphorylation.

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    Our results demonstrated that high periostin expression is a strong prognostic factor in lung cancer, and that periostin secreted by adjacent fibroblasts may promote lung cancer proliferation and invasion. We believe that periostin represents a potential target in lung‑cancer tumor progression.

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