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  P1.03 - Biology (Not CME Accredited Session) (ID 935)

  • Event: WCLC 2018
  • Type: Poster Viewing in the Exhibit Hall
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/24/2018, 16:45 - 18:00, Exhibit Hall

  • 26427

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    P1.03-29 - An Intronic Variant in EGFR Regulates GBAS Expression and Predicts Survival in Early-Stage Non-Small Cell Lung Cancer (ID 11953)

    16:45 - 18:00  |  Author(s): Mi Jeong Hong
    • Abstract
    • Slides

    Background
    

    Genome-wide association studies (GWAS) have indicated that most of the currently identified disease- and trait-associated single nucleotide polymorphisms (SNPs) are intronic or intergenic. RegulomeDB is a recently developed database that provides functional annotations for regulatory features of SNPs located in non-coding regions.

    a9ded1e5ce5d75814730bb4caaf49419 Method
    

    We evaluated the potential regulatory SNPs in the EGFR gene region using RegulomeDB and their associations with prognosis after surgery in non-small cell lung cancer (NSCLC) patients. A total of 698 patients with surgically resected NSCLC were enrolled and seven SNPs were selected based on RegulomeDB database. All SNPs were genotyped using SEQUENOM’s MassARRAY® iPLEX assay.

    4c3880bb027f159e801041b1021e88e8 Result
    

    Among the seven SNPs evaluated, the rs9642391 (EGFR ivs19+2851C>G) was found to be significantly associated with survival outcomes (adjusted HR for overall survival = 0.70, 95% CI = 0.56-0.87, P = 0.001; and adjusted HR for disease free survival = 0.82, 95% CI = 0.70-0.97, P = 0.02; under codominant model). According to RegulomeDB, the rs9642391C>G, which is located in intron 19 of EGFR, was predicted to influence the expression of glioblastoma amplified sequence (GBAS), but not EGFR. As predicted, the rs9642391C>G was associated with GBAS mRNA expression (P=0.024), but not EGFR mRNA expression in tumor tissues.

    8eea62084ca7e541d918e823422bd82e Conclusion
    

    In conclusion, our study provided evidence that the rs9642391C>G in the intron of EGFR is associated with GBAS expression and survival outcomes of patients with surgically resected early-stage NSCLC.

    6f8b794f3246b0c1e1780bb4d4d5dc53

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