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Yanfang Guan

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    P1.03 - Biology (Not CME Accredited Session) (ID 935)

    • Event: WCLC 2018
    • Type: Poster Viewing in the Exhibit Hall
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 9/24/2018, 16:45 - 18:00, Exhibit Hall
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      P1.03-11 - Analysis of T-Cell Repertoires in Benign and Malignant Solitary Pulmonary Nodules to Evaluate Tumor Immune Microenviroment (ID 13175)

      16:45 - 18:00  |  Author(s): Yanfang Guan

      • Abstract
      • Slides


      The identification of malignancy of solitary pulmonary nodule (SPN) is important for lung cancer eraly detection. Carcinogenesis may result from accumulation of molecular evolution and escaping from host immunoediting. However, the immune landscape such as T cell repertoire of benign and malignant SPNs have not been systemically studied.

      a9ded1e5ce5d75814730bb4caaf49419 Method

      We have collected resected solitary pulmonary nodules (SPNs) and peripheral blood including benign SPNs (N=10), LUSC (N=11) and LUAD (N=38) from
59 patients. T cell repertoire of infiltrating T cells in SPNs were sequenced their T cell receptor b genes (TCRb) , as well as their abundance in peripheral blood. Enrichment clonotypes were defined as 500 fold enrichment as compared to blood.

      4c3880bb027f159e801041b1021e88e8 Result

      Comparison to the peripheral blood, T cell repertoire diversity of infiltrating T cells in SPNs showed significant differences among benign and malignant. T cell clonality was higher in the benign SPNs than Malignant, however no significant differences among LUSC and LUAD. Additionally, we observed that Tumors from smokers had higher T cell clonality than no-smokers as reported earlier. Interestingly, many T cell clones, including major clones, were shared between lung tissues and matched peripheral blood; furthermore, the higher shared clonotypes of T cell clones between lung tissues and blood in benign SPNs than Malignant. Enrichment analysis demonstrated more enriched clonotypes observed in LUSC and LUAD.

      8eea62084ca7e541d918e823422bd82e Conclusion

      Our preliminary data demonstrate the distinct immune microenvironment in SPNs may be associated with the benign and malignant features. And implies a significant proportion of infiltrating T cells in SPNs may be a function of constant lung infiltrating rather than an anti-tumor response.


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