Author of

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  Poster Display session (Friday) (ID 65)

  • Event: ELCC 2018
  • Type: Poster Display session
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 4/13/2018, 12:30 - 13:00, Hall 1

  • 25365

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    164P - Feasibility of liquid biopsy using plasma and platelets for detection of ALK rearrangements in non-small cell lung cancer (ID 297)

    12:30 - 13:00  |  Author(s): H. Cho
    • Abstract
    • Slides

    Background:
    The aim of this study is to investigate the feasibility of liquid biopsy using plasma and platelets for detection of ALK rearrangement.
    
    Methods:
    FISH was performed in 664 patients between January 2015 and May 2017. We retrospectively analyzed the FFPE tissue and blood sample to detect ALK rearrangement using multiplex RT-PCR from 30 advanced NSCLC patients who had available tissue specimen. Total RNA were extracted from FFPE cell blocks, plasma and platelets, respectively. EML4-ALK fusion RNA was detected using PANAqPCR[TM] EML4-ALK fusion gene detection kit.
    
    Results:
    28 patients were FISH positive and 2 were negative. In a validation data compared with FISH, RT-PCR using FFPE tissue demonstrated 57.1% sensitivity and 69.2% accuracy. Liquid biopsy (plasma or platelets-positive) had higher sensitivity (96.4%) and accuracy (93.3%). Among the specimen of liquid biopsy, platelets showed slightly higher sensitivity and accuracy than plasma (82.1 and 83.3% vs 78.6 and 76.7%). Compared with FFPE tissue using RT-PCR, liquid biopsy showed 100% sensitivity, 20.0% specificity and 69.2% accuracy. Median proportion of positive cells in FISH was higher in subgroups of liquid biopsy with positive result (Plasma, 30.0 vs 15.0%, p = 0.062; Platelets 30.0 vs 20.0%, p = 0.104). In 18 patients with crizotinib treatment, platelets-positive subgroup showed a tendency of longer duration of treatment (7.2 vs 1.5 months, p = 0.071) and higher response rate (57.1 vs 0.0%, p = 0.092). However, platelets-positive subgroup showed significantly higher disease control rate than platelets-negative subgroup when they were treated with crizotinib (85.7 vs 25.0%, p = 0.044).
    
    Conclusions:
    Plasma and platelets are valuable sources for liquid biopsy using RT-PCR technique in detection of ALK rearrangement, and could play a supplementary role in diagnosis of ALK-positive NSCLC. Furthermore, platelets may be useful for predicting the treatment outcome of crizotinib.
    
    Clinical trial identification:
    
    
    Legal entity responsible for the study:
    Legal entity responsible for the study
    
    Funding:
    Has not received any funding
    
    Disclosure:
    All authors have declared no conflicts of interest.
    
    

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