Author of

• 25520

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  Poster Display session (Friday) (ID 65)

  • Event: ELCC 2018
  • Type: Poster Display session
  • Track:
  • Presentations: 1
  • Moderators:
  • Coordinates: 4/13/2018, 12:30 - 13:00, Hall 1

  • 25312

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    90P - Study of intestinal flora differences between small cell lung cancer (SCLC) patients and healthy people (ID 390)

    12:30 - 13:00  |  Presenting Author(s): L. Zhang
    • Abstract

    Background:
    SCLC accounts for approximately 14% of lung cancer. There are a large number of symbiotic microorganisms in the intestinal tract of the human body. Under normal circumstances, intestinal flora, the body and the external environment maintain a relative balance to maintain the health of the body. If the balance is broken, the body will show an alteration of intestinal flora, which may cause disease.
    
    Methods:
    (1) Clinical fecal specimens collection: Fresh fecal samples were collected before the first cycle of chemotherapy and labeled as 1–14. 14 healthy people were matched. (2) Intestinal bacteria DNA extraction. (3) The changes of intestinal flora were analyzed by PCR-DGGE. Cutting of the bands with significant differences in sequencing to determine the classification of bacteria. (4) Results analysis: Quantity One software and SPSS19.0 software were used to analyze the results.
    
    Results:
    (1) Intestinal flora analysis in SCLC group and healthy group: The diversity index and abundance of the healthy group was higher than those of the SCLC group, the difference in diversity index was statistically significant (P < 0.05), but the difference in abundance was not significant (P > 0.05). (2) Sequencing results analysis: Brightness of bands 1, 3, 7 and 8 were higher in the SCLC group than in the healthy group; brightness of bands 2, 4, 5, 6 and 9 were higher in the SCLC group than in the healthy group. The results show that the sequence similarity of bands 1 and 3 with Eubacterium xylanophilumisis were 98% and 97%, respectively; the sequence similarity of bands 2 and 6 with Prevotella copri 91% and 97%, respectively; the sequence similarity of band 4 with Blotia sp.was 93%; the sequence similarity of band 5 with Dialister succinatiphilus was 92%; the sequence similarity of band 7 with Eubacterium eligens was 100%; the sequence similarity of band 8 with Clostridium sp. was 99%; the sequence similarity of band 9 with Pseudobutyrivibriorum inisis 100%.
    
    Conclusions:
    (1) Intestinal flora differ between SCLC patients and healthy people, the difference in diversity index is significant, but the difference in abundance is not significant. (2) The increase of Eubacterium xylanophilum, Eubacterium eligens and Clostridium sp.are positively correlated with the occurrence of SCLC. The decrease of Prevotellacopri and Pseudobutyrivibriorum inis are positively correlated with the occurrence of SCLC.
    
    Clinical trial identification:
    
    
    Legal entity responsible for the study:
    2nd Hospital Affiliated to Dalian Medical University
    
    Funding:
    Has not received any funding
    
    Disclosure:
    All authors have declared no conflicts of interest.