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M. Van Seijen
Poster Display session (Friday) (ID 65)
- Event: ELCC 2018
- Type: Poster Display session
- Presentations: 1
- Coordinates: 4/13/2018, 12:30 - 13:00, Hall 1
21P - Influence of delayed and prolonged fixation on the evaluation of immunohistochemical staining on pulmonary resection specimen (ID 539)
12:30 - 13:00 | Presenting Author(s): M. Van Seijen
Pre-analytical factors as fixation time has influence on morphology of diagnostic and predictive immunohistochemical staining and are increasingly used in the evaluation of lung cancer. Our aim was to investigate if variations in fixation time influence the outcome of immunohistochemical staining in lung cancer.
From lung cancer resections specimen with tumor size >4 cm, 10 fragments were sampled: 2 received standard fixation, 5 delayed, and 3 prolonged fixation. After paraffin embedding, tissue microarrays (TMA) were made. Immunohistochemistry (IHC) of 20 antibodies were applied and scored for quality and intensity of staining. TMA slides were scored by core/case: quality of cores and IHC intensity in cores of sufficient quality.
Tissue with delay in start of fixation showed deterioration of tissue quality leading to reduction in the evaluation of staining for CK-7, Keratin MNF116, Cam5.2, CK5/6, TTF-1, CMET, Napsin-A, D2-40, PD-L1 antibodies. Prolonged fixation had no influence on the performance of immunohistochemical stains.
Delay of fixation negatively effects the evaluation of IHC staining. Therefore, tissue should be fixated soon after surgery.
Clinical trial identification:
Legal entity responsible for the study:
European Thoracic Oncology Platform (ETOP)
VU University Medical Center
All authors have declared no conflicts of interest.
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