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D. Xiong



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    P1.02 - Biology/Pathology (ID 614)

    • Event: WCLC 2017
    • Type: Poster Session with Presenters Present
    • Track: Biology/Pathology
    • Presentations: 1
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      P1.02-062 - Ring Finger Protein 38 Promote Non-Small Cell Lung Cancer Progression by Endowing Cell EMT Phenotype (ID 8774)

      09:30 - 16:00  |  Author(s): D. Xiong

      • Abstract
      • Slides

      Background:
      RNF38, as an E3 ubiquitin ligase, plays an essential role in multiple biological processes by controlling cell apoptosis, cell cycle and DNA repair, .this study set out to investigate the and clinical implications of Ring finger protein 38 (RNF38) in non-small cell lung cancer (NSCLC).

      Method:
      We examined RNF38 expression pattern in in multiple cancers by Oncomine expression analysis,Immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot were used to detect the levels of RNF38 protein and mRNA in NSCLC and corresponding paratumorous tissues.After RNF38 was knocked down using small hairpin RNAs (shRNA) in NSCLC cell lines (A549 and 95D), Wound-healing assays and trans-well assays were used to assess cell migration and invasion ability. Colony formation assays and CCK8 were used to detect proliferative abilities. The analysis of epithelial-to-mesenchymal transition (EMT) phenotype was carried out by immunofluorescence staining and western blot.

      Result:
      Our data revealed that elevated expression of RNF38 were more common in NSCLC tissue than paired normal tissues in both mRNA (2.82 ± 0.29 vs. 1.23 ± 0.13) and protein (2.75 ± 0.09 vs. 1.24 ± 0.02) level. Higher levels of RNF38 expression were significantly associated with lymph node metastases, higher TNM stages (p=0.011), larger tumor size (p=2.09E-04) and predicted poor prognosis. We also observed that RNF38 expression was inversely correlated with E-cadherin expression (P= 0.025). Moreover, downregulation of RNF38 in NSCLC cells, the proliferation, metastatic and invasive abilities were significantly impaired. In addition, aberrant RNF38 expression could modulate the key molecules of EMT.

      Conclusion:
      Our results indicate that elevated expression of RNF38 is significantly associated with the proliferation and metastatic capacity of NSCLC cells, and RNF38 overexpression can serve as a biomarker of NSCLC poor prognosis.

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