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P1.02 - Biology/Pathology (ID 614)
- Event: WCLC 2017
- Type: Poster Session with Presenters Present
- Track: Biology/Pathology
- Presentations: 1
- Coordinates: 10/16/2017, 09:30 - 16:00, Exhibit Hall (Hall B + C)
P1.02-042 - Circadian Clock Gene Per2 Over-Expression Inhibits Tumor Progression in Human Non-Small Cell Lung Cancer (ID 8110)
09:30 - 16:00 | Presenting Author(s): Li Qiang
The Period clock gene family has three family members, including Per1, Per2 and Per3. Among them, Per2 is an indispensable component of the circadian clock, which not only modulates circadian oscillations, but also has a decreased expression in many types of cancers and functions as a tumor suppressor gene. However, the expression and specific roles of Per2 in human non-small cell lung cancer(NSCLC) is still unknown. And the present study is aimed to investigate the roles of Per2 porced expression in NSCLC in vitro and in vivo.
Per2 expression level in tissues was examined by immunohistochemistry. mRNA and protein expression alterations were teste by RT-PCR and Western blot. To clarify the specific roles of Per2 in A549 cell line, we construct the stable overexpressed cell line by lentivirus transfection. And the roles of Per2 porced expression in tumor proliferation and migration were examined by CCK8, colony formation, cell wound, cell migration and invasion, and we also detected the alterations in cell cycle and apoptosis by flow cytometric analysis. Furthermore, we established subcutaneous tumor animal mode to test the tumorgenesis and migration ability of Per2 overexpressed A459 cell in vivo.
We found that Per2 has a commonly higher expression in para-carcinoma tissues than carcinoma tissues in 26 NSCLC patients(P=0.0001). And Per2 expression in NSCLC patients were correlated with some clinicopathological features(P=0.0000). Then, function assay showed that forced expression of Per2 in A549 cells markedly decreased the ability of cancer cell proliferation, migration and invasion in vitro(P<0.05), and also increased the apoptosis and the number of cells in G1/G0 phase(P<0.05). Furthermore, overexpression of Per2 altered the proliferation and migration related protein expression(P<0.05). Consistent with the in vitro study, we also showed that Per2 overexpression decreased the tumorigenicity of A549 cells in vivo(P<0.05).
Per2 has a lower expression in NSCLC tissues and also funtions as a tumor supressor gene. It regulates the numerous important downstream tumor-related genes, which may be a novel molecular target for cancer treatment.