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D. Dimitrova



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    Poster Display Session (ID 63)

    • Event: ELCC 2017
    • Type: Poster Display Session
    • Track:
    • Presentations: 1
    • Moderators:
    • Coordinates: 5/07/2017, 12:30 - 13:00, Hall 1
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      150P - Immunohistochemical characteristics of brain metastases and corresponding primary lung cancer (ID 360)

      12:30 - 13:00  |  Author(s): D. Dimitrova

      • Abstract

      Background:
      About 40% of patients with lung cancer (LC) develop brain metastases (BM). The prognosis is poor. The study of BM is important for better understanding of the biology of LC.

      Methods:
      Surgically resected BMs and corresponding primary LCs from 30 patients (men n = 25, 83%; age 55±9) were studied: adenocarcinoma (AC)-21, squamous cell carcinoma (SCC)-5, small cell lung carcinoma (SCLC)-4. The histological subtype and immunohistochemical expression of TTF-1, p63, cytokeratin 7, synaptophysin, Ki-67 (proliferative activity) and CD31 (number intratumoral microvessels-NIM) were evaluated.

      Results:
      The histology of LC compared with BM is different in half of the AC and without difference in SCC and SCLC. ACs are mainly with acinar (53% cases) and solid (37%) components. Metastatic ACs are more often with papillary (47%) component. In 47% of AC BM has different histological structure than LC. The acinar AC are predominantly papillar in 70% of BM showing that the papillary component metastasize most frequently. TTF-1 is expressed in greater number of lung AC (n = 20, 95%), but with lower mean levels of expression, while the corresponding BM express the marker less frequently (n = 16, 76%), but when it is presented it has higher mean values of expression (45.44 vs73.88, p = 0.011). P63 is expressed in high percentage in all SCC (n = 5,100%); there is no difference in expression between LC and BM (80.6 vs 81.6, p = 0.68). Cytokeratin 7 is expressed in all AC equally regardless of the site - primary or metastatic. Ki-67 proliferative index (PI) is higher in SCLC than in lung AD (p = 0.008), in SCLC BM than in AD BM (p < 0.001), in SCLC BM than in SCC BM (p = 0.008). It was found that the Ki-67 PI BM is higher than that of AC LC (30 vs. 17, p = 0.003), in SCC (35 vs.27, p = 0.048) but without difference in SCLC (p = 0.141). CD31 establish vascular invasion in LC – NIM is higher in AC than in SCLC (55vs.31, p = 0.003), in SCC than in SCLC (61vs.31, p = 0.009), no difference between AC and SCC (66 vs.61, p = 0.467). There were no significant differences between LC and BM.

      Conclusions:
      There are differences between primary LC and corresponding BM – in histology structure, in immunohistochemical expression, and in proliferative activity.

      Clinical trial identification:


      Legal entity responsible for the study:
      Medical University, Sofia.

      Funding:
      Supported by Grant 360/2015-Contract Nr.76/2015 funded by Medical University, Sofia.

      Disclosure:
      All authors have declared no conflicts of interest.