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T.F. Lv
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P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)
- Event: WCLC 2015
- Type: Poster
- Track: Biology, Pathology, and Molecular Testing
- Presentations: 1
- Moderators:
- Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)
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P1.04-087 - The Role and Mechanism of Twist1 in Non-Small Cell Lung Cancer Pathogenesis (ID 1215)
09:30 - 17:00 | Author(s): T.F. Lv
- Abstract
Background:
Metastasis is a multistep process and the main cause of disease failure and mortality in lung cancer patients. Twist1 is a highly conserved developmental gene involved in embryogenesis that could be reactivated in cancers promoting both malignant conversion and cancer progression through epithelial-mesenchymal transition (EMT). The aim of this study was to investigate the role and mechanism of Twist1 in the pathogenesis of lung cancer.
Methods:
We examined a series of surgical lung cancer samples from Chinese patients (n=75) and showed that Twist1 expression was linked to lymph node status (P<0.05). To validate that Twist1 is a driver of EMT in non-small cell lung cancer (NSCLC), we used two human lung cancer cell lines (H1650 and H1975, EGFR mutation) and demonstrated that Twist1 was associated with cell growth and mobility.
Results:
Overexpression of Twist1 increased cell growth, mobility, and a decrease of Twist1 by shRNA technology reversed the phenomenon. Twist1 promoted the tumor growth in vivo and induced the expression changes of many genes by tumor gene RNA array. Twist1 significantly down-regulated p4EBP1 expression in H1650 cells and up-regulated p4EBP1 in H1975 cells by qRT-PCR and western blot assay.
Conclusion:
Collectively, both our in vivo and in vitro findings support that Twist1 in promoting lung cancer by upregulation p4EBP1, which are needed to further study the role of Twist1in NSCLC
