Author of

• 13580

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  P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 13665

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    P1.04-085 - The Role and Potential Mechanisms of LncRNA-TATDN1 in the Metastasis and Invasion of 95D Cell (ID 1675)

    09:30 - 17:00  |  Author(s): N. Zequn
    • Abstract

    Background:
    The invasion and metastasis of malignant cell is generally considered as a major reason why it is always failed in the therapy of lung cancer. The mechanism in metastasis is complicated and uncertain as well. The scientific research proves that Long non-coding RNA (LncRNA) is bound up with the occurrence, development and prognoses of lung cancer.
    
    Methods:
    Application of high throughput LncRNA chip to investigate the differentially expressed LncRNA between 95D and 95C cell lines. RNA interference (RNAi) approaches were used for the analysis of the biological functions and metastasis of TATDN1. Tumor growth was studied in nude mice.
    
    Results:
    TATDN1-1 was highly expressed in 95D cells lines and NSCLC tissues. In 95D cells knockdown of TATDN1 by using small interfering RNA (siRNA) resulted in significant reduction in proliferation, adhesion, migration and invasion of these cells in vitro. 95D cells xenografts with decreased TATDN1 expression were impaired in tumor formation and growth. On genetic level, MALAT-1displays the strongest association with genes involved in cancer like cellular growth, movement, proliferation, signaling.
    
    Conclusion:
    These data indicate that TATDN1expression levels are associated with 95D cells metastasis and identify tumor-promoting functions of TATDN1.