Author of

• 13580

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  P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 13664

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    P1.04-084 - Ubiquitin Protein Ligase E3C Promotes Growth and Metastasis of Non-Small Cell Lung Cancer (ID 1206)

    09:30 - 17:00  |  Author(s): D. Ge
    • Abstract

    Background:
    Ubiquitin protein ligase E3C (UBE3C) has been recently proposed as a potential oncogene in hepatocellular carcinoma. However, its role and mechanism in non-small cell lung cancer remains unknown.
    
    Methods:
    Eight cases of NSCLC and matched nontumorous samples were used to analyze UBE3C expression at the level of protein. Then, we down-regulated the expression of UBE3C in NSCLC cells and assessed the biological role of UBE3C in NSCLC cell line. Finally, the prognostic role of UBE3C was examined by Immunohistochemistry (IHC) in tissue microarray (TMA) consisting of 208 cases of NSCLC.
    
    Results:
    The expression of UBE3C in NSCLC tissues was much higher than that in nontumorous samples. Downregulation of UBE3C expression suppressed the proliferation and invasion of lung cancer cells. Further analysis showed that downregulation of UBE3C expression mainly promoted cell apoptosis but without an effect on cell cycle. High levels of UBE3C expression were associated with higher tumor stage in NSCLC patients. The 5-year overall survival rate in the UBE3C[high] group was significantly lower than that in the UBE3C[low] group. In multivariate analysis, UBE3C was identified as an independent prognostic factor for overall survival.
    
    Conclusion:
    Our findings indicated that UBE3C may represent a candidate therapeutic target and a novel prognostic marker of NSCLC.