Author of

• 13580

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  P1.04 - Poster Session/ Biology, Pathology, and Molecular Testing (ID 233)

  • Event: WCLC 2015
  • Type: Poster
  • Track: Biology, Pathology, and Molecular Testing
  • Presentations: 1
  • Moderators:
  • Coordinates: 9/07/2015, 09:30 - 17:00, Exhibit Hall (Hall B+C)

  • 13663

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    P1.04-083 - The Metastasis-Related Noncoding RNA Expression Profile and LncRNA LOC101448202 Induces 95D Cell Migration and Tumor Growth (ID 1640)

    09:30 - 17:00  |  Author(s): W. Guoen
    • Abstract

    Background:
    Metastasis of non-small cell lung cancer shortens the survival time of patients and decreases their quality of life. This unfortunate situation could be improved if the functions of long noncoding RNAs (lncRNAs) were identified in depth.
    
    Methods:
    not applicable
    
    Results:
    In the present study, a large number of lncRNAs and mRNAs with different expression patterns were verified in 95D and 95C lung cancer cell lines. The lncRNA, LOC101448202, was highly expressed in 95D cells, and lentivirus-mediated RNA interference was able to silence its expression with a silencing efficiency of 92%. LOC101448202 gene silencing led to a decrease in cell proliferation, adhesion, migration, and invasion and the number of pseudopods and microvillion the cell surface was also reduced. At the mRNA level, her-2 expression was inhibited and the expression of nm23-H1 and E-cadherin was increased. At the protein level, β-catenin and ezrin levels were decreased both in vitro and in vivo. In clinical specimens and mouse models, LOC101448202 expression was positively related to tumor growth.
    
    Conclusion:
    These data indicate that LOC101448202 expression levels are associated with 95D cell metastasis, demonstrating the tumor-promoting function of this lncRNA.